Amelioration of dextran sulfate sodium-induced ulcerative colitis by fermented Lycium barbarum polysaccharides through modulation of intestinal microecology
To explore the mechanism of the intestinal microecology regulation by polysaccharide prebiotics,ELISA,histopathologic analysis,immunohistochemical analysis,16S rRNA high-throughput sequencing,and gas chromatography-mass spectrometry were applied to investigate the effects of fermented polysaccharides on changes in the intestinal microbiota and short-chain fatty acids(SCFAs)in mice with dextran sulfate sodium(DSS)-induced colitis model and their relationship with the level of intestinal inflammation and barrier protein expression.It was found that fermented Lycium barbarum polysaccharides(FLBP)significantly reduced intestinal inflammation level,improved colonic tissue structure,up-regulated the expression of tight junction proteins Claudin-1 and ZO-1,and significantly increased the content of intestinal SCFAs in mice.Gut bacteria analyses showed that FLBP enriched intestinal Dubosiella and Akkermansia in mice and decreased the abundance of Turicibacter,Faecalibaculum,and Escherichia-Shigella.Results showed that remodeled Dubosiella activated by FLBP played a dominant role in ameliorating colitis by significantly increasing SCFAs content,improving intestinal barrier and reducing intestinal inflammation.The study aimed to provide a safer and better option for the amelioration of colitis and to provide a theoretical basis for the development of functional foods with FLBP.
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