Ⅱ型鼠李半乳糖醛酸聚糖的制备方法和结构表征的研究进展
Advances in the preparation and structural characterization of rhamnogalacturonan Ⅱ
刘育朋 1施松善 1步盈萱 1龚欢 1王辉俊 2王顺春2
作者信息
- 1. 上海中医药大学中药研究所,上海 201203
- 2. 上海中医药大学中药研究所,上海 201203;中药标准化教育部重点实验室,上海 201203
- 折叠
摘要
Ⅱ型鼠李半乳糖醛酸聚糖(RG-Ⅱ)是果胶结构域中的一种,其结构在不同物种之间具有高度的保守性.RG-Ⅱ的主链由约 9个半乳糖醛酸经α-1,4糖苷键连接而成,并被 6个(A-F)明确定义的侧链取代.其中二糖侧链C、D和单糖侧链E、F的结构在不同来源的RG-Ⅱ中基本保持相同.寡糖侧链A和B则存在轻微的变异性.通过相对分子质量、单糖组成和质谱分析等手段可实现RG-Ⅱ的结构表征.中药中含有RG-Ⅱ结构域的多糖具有很高的药用价值,使用内切多聚半乳糖醛酸酶(Endo-PG)和草酸青霉可将RG-Ⅱ从中药中分离并对其在多糖中的含量进行测定.从葡萄酒中可快速制备大量RG-Ⅱ标准品用于新定量方法的开发,实现对中药活性多糖的质量控制,推动中药多糖的研究进程.
Abstract
Rhamnogalacturonan Ⅱ(RG-Ⅱ)is one of the structural domains of pectin whose structure is highly conserved among species.The main chain of RG-Ⅱ consists of approximately nine galacturonic acids linked by α-1,4 glycosidic bonds,with six well-defined side chains replacing them(A-F).The structures of the disaccharide side chains C and D and the monosaccharide side chains E and F in RG-Ⅱ from different sources remain essentially the same.In contrast,the oligosaccharide side chains A and B showed slight variability.Structural characterization of RG-Ⅱ can be achieved by molecular weight,monosaccharide composition,and mass spectrometry.The polysaccharides containing RG-Ⅱ structural domains in traditional Chinese medicines(TCMs)have high medicinal value.Isolation of RG-Ⅱ can be achieved using endo-polygalacturonase(Endo-PG)and Penicillium oxalicum.A substantial number of RG-Ⅱ standards can be rapidly prepared from red wine for the development of new quantitative methods to realize the quality control of active polysaccharides from traditional Chinese medicines and to promote the research process of polysaccharides from traditional Chinese medicines.
关键词
果胶/Ⅱ型鼠李半乳糖醛酸聚糖/RG-Ⅱ/内切多聚半乳糖醛酸酶/草酸青霉/葡萄酒Key words
pectin/rhamnogalacturonan Ⅱ/RG-Ⅱ/endo-polygalacturonase/penicillium oxalate/red wine引用本文复制引用
基金项目
上海市自然科学基金(21ZR1462900)
国家自然科学基金(82274078)
国家自然科学基金(U23A20512)
上海市东方英才计划青年项目()
Shanghai Oriental Talent Program for Youth()
出版年
2024
国家科技期刊平台
NSTL
万方数据