In this study,a new in situ film of compound iodine oral spray was prepared by in situ gel technology,which was used to exert sustained-release effect for promoting the repair of oral ulcer wounds.Firstly,the formulation and process of the spray solution were optimized according to the spray state and film-forming time.The drug-liquid mixing ratio was evaluated by film-forming time and drug film adhesion.The drug content,stability,pH,and spraying effect of compound iodine oral spray prepared by the optimal formulation were investigated;and the physicochemical properties,including film formation time,solubility,hygroscopicity,moisture retention and in vitro release of drug film were evaluated.In addition,the biocompatibility of the film-forming materials and proliferation ability of drug film were investigated by cell experiment.Through the rabbit oral ulcer model,the in vivo film-forming and repair-promoting effects of compound iodine oral spray were evaluated.The results showed that the pH of liquid A and liquid B prepared were 6.21±0.02 and 6.42±0.03,respectively,which were in line with the normal pH range of oral mucosa;liquid A and liquid B had good stability and spray state;the iodine content in solution B was(1.96±0.01)mg/mL;the in situ membrane formation time in vitro and in the oral cavity were(118.3±3.6)s and(133.3±4.6)s,respectively;the 24-hour dissolution rate was(87.31±1.74)％,the moisture absorption rate was(124.17±7.13)％,and the moisture retention rate was(26.85±2.50)％;the iodine content in the oral spray was(47.42±0.39)mg/g,with good flexibility and adhesion,as well as some slow-release effect.In cell experiment,the film-forming materials showed good biocompatibility and growth promotion ability.The results of the rabbit oral ulcer experiment showed that the compound iodine oral spray could rapidly form a film in vivo and significantly promote the repair of oral ulcer.In conclusion,the compound iodine oral spray in situ film with a stable preparation process can effectively promote the repair of oral ulcer wounds,which provides a new idea for the research of novel oral mucosa formulations,with a good prospect of transfer.
关键词
口腔喷雾原位膜/复方碘/口腔溃疡/缓释/促修复
Key words
oral spray in situ membrane/compound iodine/oral ulcer/sustained release/promoting repair
维普
万方数据