Preparation and evaluation of in situ film of compound iodine oral spray for promoting oral ulcer repair
In this study,a new in situ film of compound iodine oral spray was prepared by in situ gel technology,which was used to exert sustained-release effect for promoting the repair of oral ulcer wounds.Firstly,the formulation and process of the spray solution were optimized according to the spray state and film-forming time.The drug-liquid mixing ratio was evaluated by film-forming time and drug film adhesion.The drug content,stability,pH,and spraying effect of compound iodine oral spray prepared by the optimal formulation were investigated;and the physicochemical properties,including film formation time,solubility,hygroscopicity,moisture retention and in vitro release of drug film were evaluated.In addition,the biocompatibility of the film-forming materials and proliferation ability of drug film were investigated by cell experiment.Through the rabbit oral ulcer model,the in vivo film-forming and repair-promoting effects of compound iodine oral spray were evaluated.The results showed that the pH of liquid A and liquid B prepared were 6.21±0.02 and 6.42±0.03,respectively,which were in line with the normal pH range of oral mucosa;liquid A and liquid B had good stability and spray state;the iodine content in solution B was(1.96±0.01)mg/mL;the in situ membrane formation time in vitro and in the oral cavity were(118.3±3.6)s and(133.3±4.6)s,respectively;the 24-hour dissolution rate was(87.31±1.74)％,the moisture absorption rate was(124.17±7.13)％,and the moisture retention rate was(26.85±2.50)％;the iodine content in the oral spray was(47.42±0.39)mg/g,with good flexibility and adhesion,as well as some slow-release effect.In cell experiment,the film-forming materials showed good biocompatibility and growth promotion ability.The results of the rabbit oral ulcer experiment showed that the compound iodine oral spray could rapidly form a film in vivo and significantly promote the repair of oral ulcer.In conclusion,the compound iodine oral spray in situ film with a stable preparation process can effectively promote the repair of oral ulcer wounds,which provides a new idea for the research of novel oral mucosa formulations,with a good prospect of transfer.
oral spray in situ membranecompound iodineoral ulcersustained releasepromoting repair
万方数据
维普
