低钾胁迫下过表达NtCaM10对烟草生长及钾素吸收的影响
Effect of Overexpression of NtCaM10 on Tobacco Growth and Potassium Absorption under Low Potassium Stress
刘灿 1代晓燕 1张玉银 1王英锋 1后亚斌 1李莹莹 1杨杰 1夏宗良 2常剑波3
作者信息
- 1. 河南农业大学烟草学院,国家烟草行业烟草栽培重点实验室,郑州 450002
- 2. 河南农业大学生命学院,郑州 450002
- 3. 河南省烟草公司三门峡市公司,河南三门峡 472000
- 折叠
摘要
为探究低钾胁迫下过表达NtCaM10基因对烟草生长及钾素吸收的生理与分子调控机制,以野生型烟草K326(WT)及过表达NtCaM10转基因K326为试验材料,设置两个钾水平,5 mmol/L(常钾)、0.15 mmol/L(低钾),分析低钾胁迫下NtCaM10基因的表达模式及启动子活性,测定相关生理生化指标、钾离子吸收相关基因的相对表达量及各部位钾含量.结果表明,低钾胁迫后NtCaM10基因的相对表达量显著上升、启动子活性被激活.与常钾水平相比,低钾水平下WT和转基因株系的鲜质量及根系长度均显著降低.在低钾水平下,WT生长受抑制的效果更加明显,NtCaM10过表达 3个转基因株系的平均鲜质量相比WT显著提高了 57.44%,平均根系长度显著提高了 35.77%,转基因系中丙二醛的积累显著低于WT,SOD、POD、CAT的活性均显著高于WT,NtCaM10过表达烟草中的NtHAK1、NtHAK5、NtKC1、NtNKT2的平均相对表达量显著上调,增幅分别为 56.60%、81.84%、98.08%、638.89%,过表达NtCaM10烟草根部和地上部的平均钾素积累与WT相比显著提高了 21.59%和 32.36%.NtCaM10基因参与烟株对低钾胁迫的响应,过表达NtCaM10可以通过调节烟草的抗氧化酶活性和钾离子吸收与转运相关基因的表达来正向调节烟草对低钾胁迫的耐受性.
Abstract
This research aims to explore the physiological and molecular mechanisms involved in overexpression of NtCaM10 gene on growth and potassium uptake of tobacco under low potassium stress.The experiment adopted wild type(WT)K326 and overexpression of NtCaM10 transgenic tobacco as experimental materials and consisted of two potassium levels,i.e.5 mmol/L(normal potassium)and 0.15 mmol/L(low potassium).The expression pattern and promoter activity of NtCaM10 gene under low potassium stress were analyzed.The physiological and biochemical indexes,the relative expression of genes related to potassium uptake and the content in different parts of tobacco plants under low potassium stress were determined.The results showed that the relative expression of NtCaM10 gene was significantly increased and the promoter activity was highly initiated after low potassium stress.Under low potassium level,the fresh weight and root length were significantly reduced in WT or transgenic lines compared to the control,and the WT growth was evidently hindered.Compared to WT,the fresh weight and root length of three transgenic lines overexpressing NtCaM10 were significantly increased by 57.44%and 35.77%,respectively.In the transgenic lines,the accumulation of MDA was significantly lower,while the activities of SOD,POD,and CAT were significantly higher than WT.Compared with WT,the average relative expression levels of NtHAK1,NtHAK5,NtKC1 and NtNKT2 in NtCaM10 overexpressed tobacco were significantly upregulated,and increased by 56.60%,81.84%,98.08%and 638.89%,respectively.Whereas,the potassium accumulation in root and shoot of tobacco overexpressed NtCaM10 was significantly increased by 21.59%and 32.36%compared to WT.The NtCaM10 gene is involved in the response of tobacco plants to low-potassium stress,and overexpression of NtCaM10 can positively regulate tolerance of tobacco to low-potassium stress by modifying the activities of antioxidant enzymes and the expression of genes related to potassium absorption and transport.
关键词
烟草/低钾胁迫/NtCaM10/钾素吸收Key words
tobacco/low potassium stress/NtCaM10/potassium absorption引用本文复制引用
基金项目
国家自然科学基金(31501823)
河南省烟草公司洛阳市公司科技专项(2021410300270050)
河南省烟草公司三门峡市公司科技专项(2022410000240015)
出版年
2024
国家科技期刊平台
NSTL
万方数据