首页|棘孢木霉与30%霜霉·嘧菌酯协同防治烟草镰刀菌根腐病

棘孢木霉与30%霜霉·嘧菌酯协同防治烟草镰刀菌根腐病

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  • 国家科技期刊平台
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为筛选防治烟草镰刀菌根腐病高效、安全的复配剂，采用菌丝生长速率法测定生防棘孢木霉Tr-0111、化学杀菌剂30%霜霉·嘧菌酯对尖孢镰刀菌(Fusarium oxysporum)的毒力及两者的相容性，同时测定两者混配对尖孢镰刀菌的毒力系数，并通过盆栽试验评价其对烟草镰刀菌根腐病的防治效果。结果表明，30%霜霉·嘧菌酯和棘孢木霉Tr-0111对尖孢镰刀菌均具有较强的毒力，其EC50 值分别为 0。0643 mL/L、2。36×102 cfu/mL，且两者相容性好。除V30%霜霉·嘧菌酯∶VTr-0111=4∶6时无增效作用，其他 8个混配比例均具有增效作用，其中V30%霜霉·嘧菌酯∶VTr-0111=7∶3时增效比率最高，为 1。16，抑菌率为68。37%。盆栽试验结果表明，V30%霜霉·嘧菌酯∶VTr-0111=5∶5时，对烟草镰刀菌根腐病的防效最好，为 78。18%，其次为 7∶3和 1∶9，防效分别为 77。27%和 72。73%，均显著高于单一使用 30%霜霉·嘧菌酯和棘孢木霉Tr-0111。因此，可以将0。0643 mL/L 30%霜霉·嘧菌酯和2。36×102 cfu/mL棘孢木霉Tr-0111以5∶5比例混匀复配应用于烟田防治烟草镰刀菌根腐病，减少化学农药使用。
Synergistic Control of Tobacco Fusarium Root Rot by Trichoderma asperellum and 30%Propamocarb·Azoxystrobin
The screening of the high-efficient and safe mixtures is of great importance for the prevention and control of tobacco Fusarium root rot.In this study,the mycelial growth rate method was used to determine the toxicity and compatibility and the toxicity coefficient of biocontrol agent Trichoderma asperellum Tr-0111 and chemical fungicide 30%propamocarb·azoxystrobin against F.oxysporum.In addition,their control effect on tobacco Fusarium root rot was evaluated through pot experiments.The results indicated that both 30%propamocarb·azoxystrobin and Trichoderma asperellum Tr-0111 had strong virulence against F.oxysporum,with EC50 values of 0.0643 mL/L and 2.36×102 cfu/mL,and showed good compatibility.The combination of 30%propamocarb·azoxystrobin and Trichoderma asperellum Tr-0111 with different ratio showed synergistic effect,except for V30%propamocarb·azoxystrobin∶VTr-0111=4∶6.The highest synergistic effect was obtained when 30%propamocarb·azoxystrobin and Trichoderma asperellum Tr-0111 was mixed at the ratio of 7∶3,with the synergistic ratio 1.16 and antibacterial rate 68.37%.The pot experiment showed that,the best control effect(78.18%)against tobacco Fusarium root rot was obtained when V30%propamocarb·azoxystrobin∶VTr-0111=5∶5,followed by 7∶3 and 1∶9,with control effects of 77.27%and 72.73%,respectively.These control effects were all significantly higher than the single use of 30%propamocarb·azoxystrobin and Trichoderma asperellum Tr-0111.Therefore,to reduce the use of chemical pesticides,the mixture of 0.0643 mL/L of 30%propamocarb·azoxystrobin and 2.36×102 cfu/mLTrichoderma asperellum Tr-0111 at the ratio of 5∶5 is recommended for the control of tobacco Fusariumroot rot.

tobacco Fusarium root rotF.oxysporum30%propamocarb·azoxystrobin suspension agentTrichoderma asperellumcompound

刘畅、李小杰、张梦丹、陈玉国、苗淑月、邱睿、白静科、尚颐茹、李淑君

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河南省农业科学院烟草研究所,烟草行业黄淮烟区烟草病虫害绿色防控重点实验室,河南许昌 461000

烟草镰刀菌根腐病 尖孢镰刀菌 30%霜霉·嘧菌酯悬浮剂 棘孢木霉 复配

上海烟草集团科技项目中国烟草总公司重大科技项目河南省农业科学院科技创新团队专项项目中国烟草总公司重大科技项目

2022310000140541110202101051LS-112022TD26110202201026LS-10

2024

10.13496/j.issn.1007-5119.2024.01.007

中国烟草科学
中国农业科学院烟草研究所 中国烟草总公司青州烟草研究所

中国烟草科学

CSTPCD北大核心
影响因子：1.318
ISSN：1007-5119
年,卷(期)：2024.45(1)
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