低NO3-胁迫抑制烟草根系质外体运输的机制研究
Mechanism of Low NO3-Stress Inhibiting Apoplastic Transport in Tobacco Roots
张标 1许耘祥 1张莉汶 1朱莹莹 2刘海伟2
作者信息
- 1. 中国农业科学院烟草研究所/农业农村部烟草生物学与加工重点实验室,青岛 266101;中国农业科学院研究生院,北京 100081
- 2. 中国农业科学院烟草研究所/农业农村部烟草生物学与加工重点实验室,青岛 266101
- 折叠
摘要
为探究低NO3-胁迫抑制烟草根系质外体运输的生理机制,以中烟100为试验材料,在水培条件下设计了 0.01、0.1 mmol/L两个低NO3-胁迫处理,及4.0 mmol/L正常NO3-浓度的对照处理,分析了各浓度NO3-对烟苗发育、根系形态、蒸腾作用、质外体途径运输、内皮层木栓化以及质外体屏障形成相关基因表达的影响.结果表明,低NO3-胁迫(0.01和0.1 mmol/L)烟苗根冠比显著大于对照,增幅分别为267％和125％,蒸腾量显著小于对照,降幅分别为176％和114％;PTS荧光强度显著小于对照,降幅分别为31％和27％,木质部伤流液以及地上部PTS浓度显著小于对照,降幅分别为105％、91％和103％、75％;低NO3-胁迫诱导烟草根内皮层的早期木栓化,木栓质沉积位置更靠近根尖;低NO3-胁迫诱导与质外体屏障形成相关基因的表达上调.综上,低NO3-胁迫诱导的内皮层木栓化抑制了烟草根系质外体途径运输.
Abstract
To explore how low NO3-stress affects the apoplastic pathway transport in tobacco root,two low NO3-stress treatments were implemented at concentrations of 0.01 and 0.1 mmol/L,while 4.0 mmol/L NO3-treatment served as the control.The flue-cured tobacco variety Zhongyan100 was employed as the experimental material.Various parameters including tobacco seedling development,root morphology,transpiration rate,apoplastic pathway transport,endodermal suberization,and gene expression related to apoplastic barrier formation were analyzed.The root/shoot of low NO3-stress(0.01 mmol/L and 0.1 mmol/L)was significantly higher than that of the control,with increases of 267％and 125％,respectively;The transpiration capacity of low NO3-stress was significantly lower than that of the control,with decreases of 176％and 114％,respectively;The PTS fluorescence intensity of low NO3-stress is significantly lower than that of the control,with the decrease of 31％and 27％,respectively.The PTS concentrations in xylem sap and shoot of low NO3-stress exhibited lower than that of the control,with the decrease of 105％,91％and 103％,75％,respectively.Moreover,low NO3-stress induced early endodermal suberization of tobacco roots,with suberin deposition observed nearer to the root tip.This stress condition meanwhile upregulated gene expression associated with apoplastic barrier formation.In summary,low NO3-stress-induced endodermal suberization inhibited the apoplastic pathway transport in tobacco roots.
关键词
低NO3-/胁迫/根/木栓化/质外体途径/PTSKey words
low NO3-/stress/root/suberization/apoplastic pathway/PTS引用本文复制引用
基金项目
山东省自然科学基金面上项目(ZR2023MC150)
中国农科院科技创新工程项目(ASTIP-TRIC03)
出版年
2024
国家科技期刊平台
NSTL
万方数据