阿南德氏链霉菌FXJ-25的鉴定及对烟草靶斑病的防效
Identification of Streptomyces anandii FXJ-25 and Its Control Efficacy Against Tobacco Target Spot Pathogen
苏家恩 1杨志娟 1董天学 2赵英佑 2王显跃 2费明亮 3陈颐 3钟杰4
作者信息
- 1. 云南省烟草公司大理市公司,云南大理 671000
- 2. 云南省烟草公司曲靖市公司,云南曲靖 655000
- 3. 云南省烟草农业科学研究院,昆明 650031
- 4. 湖南农业大学植物保护学院,长沙 410128
- 折叠
摘要
为筛选烟草靶斑病菌拮抗菌,丰富烟草靶斑病生防资源,采用稀释涂布法和平板对峙法进行放线菌分离,筛选得到一株具有明显拮抗效果的菌株FXJ-25.基于培养特性、生理生化特性及16S rDNA基因序列分析相结合的方法对该菌株进行鉴定,分析了其诱导烟草抗性的能力,并通过盆栽试验对其防效进行了研究.结果表明,拮抗菌FXJ-25对烟草靶斑病菌的抑菌率为74.14％,可引起菌丝畸形,经鉴定该菌株为阿南德氏链霉菌(Streptomyces anandii).FXJ-25具有广谱抗真菌活性,对瓜类蔓枯病菌(Stagonosporopsis cucurbitacearum)、烟草喀斯特炭疽菌(Colletotrichum karstii)和小麦赤霉病菌(Fusarium graminearum)等15种植物病原真菌均具有较好的拮抗作用,抑菌率在53.43％～100％之间.菌株发酵液还可显著提高烟苗苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)和过氧化氢酶(CAT)等防御相关酶的活性.盆栽防效中,菌株FXJ-25活菌液(108孢子/mL)对烟草靶斑病具有78.81％的防效.因此,FXJ-25可作为烟草靶斑病的生防材料进一步开发利用.
Abstract
In order to screen the antagonism of tobacco target spot pathogen and provide a new biocontrol resource for the control of tobacco target spot,strain FXJ-25 with obvious antagonistic effects was obtained by dilution plating and plate confrontation methods.This strain was identified based on the combination of culture characteristics,physiological and biochemical characteristics and 16S rDNA gene sequence analysis.The ability of strain FXJ-25 to induce tobacco resistance was analyzed,and its control effect was studied by pot control experiment.The results showed that a strain of FXJ-25 identified as Streptomyces anandii was obtained,which had an inhibitory rate of 74.14％against the pathogen of tobacco target spot and could cause mycelial malformation.FXJ-25 has broad-spectrum antifungal activities,possessing inhibitory rates of 53.43％to 100％to 15 plant pathogenic fungi including Stagonosporopsis cucurbitacearum,Colletotrichum karstii and Fusarium graminearum.The fermentation broth of this strain could also significantly induce and increase the activities of defense-related enzymes such as phenylalanine ammonia-lyase(PAL),peroxidase(POD),polyphenol oxidase(PPO),catalase(CAT)and so on.In the pot control experiment,the live bacterial solution(108 spores/mL)of strain FXJ-25 had 78.81％control efficiency on tobacco target spot.Therefore,FXJ-25 can be used as a biocontrol resource for tobacco target spot disease control for further development and utilization.
关键词
阿南德氏链霉菌/生物防治/烟草靶斑病/抑菌谱Key words
Streptomyces anandii/biological control/tobacco target spot/antimicrobial spectrum引用本文复制引用
基金项目
云南省烟草公司大理州公司科技项目一般项目(DLYC2022003)
国家自然科学基金地区科学基金(32060630)
云南省烟草农业科学研究院科技计划一般项目(2022530401641002)
湖南省自然科学基金面上项目(2023JJ30302)
出版年
2024
国家科技期刊平台
NSTL
万方数据