首页|HPLC法测定复方芩苍鼻喷雾剂中黄芩苷的含量

HPLC法测定复方芩苍鼻喷雾剂中黄芩苷的含量

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
目的:建立测定复方芩苍鼻喷雾剂中黄芩苷含量的方法.方法:采用高效液相色谱法.色谱柱为Waters Symmetry C18(150 mm×4.6 mm,5 μm),流动相为甲醇-水-磷酸(47∶53∶0.2,V/V/V),流速为1.0 ml/min,检测波长为280hm,柱温为20℃.结果:黄芩苷的质量浓度在6.2～310.0 μg/ml范围内与峰面积积分值呈良好线性关系(r=0.999 3);平均加样回收率为98.72％,RSD=0.89％(n=9).结论:本方法简便、快速、准确、重复性好,可用于复方芩苍鼻喷雾剂中黄芩苷的含量测定.
Content Determination of Baicalin in Compound Qincang Nasal Sprays by HPLC
OBJECTIVE: To establish a method for the content determination of baicalin in Compound qincang nasal sprays. METHODS: HPLC method was adopted. The determination was performed on Waters Symmetry C18(150 mm×4.6 mm,5 μrn) column with mobile phase consisted of methanol-water-phosphoric acid (47:53:0.2, V/V/V) at the flow rate of 1.0 ml/min. The detection wavelength was set at 280 nm and column temperature was 20 ℃. RESULTS: The linear range of baicalin was 6.2-310.0 μg/ml (r=0.999 3) with an average recovery of 98.72% (RSD=0.89% ,n=9). CONCLUSION: The method is simple, rapid, accurate and reproducible, and it is suitable for the content determination of baicalin in Compound qincang biyan sprays.

Compound qincang nasal spraysHPLCBaicalinContent determination

韩宪忠、金蜀蓉、柯昌毅、谢剑锋

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重庆市第三人民医院,重庆400014

复方芩苍鼻喷雾剂 高效液相色谱法 黄芩苷 含量测定

2013

10.6039/j.issn.1001-0408.2013.07.25

中国药房
中国医院协会,中国药房杂志社

中国药房

CSTPCDCSCD
影响因子：0.956
ISSN：1001-0408
年,卷(期)：2013.24(7)
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