首页|茵陈黄连汤大鼠离体肠道渗透机制研究

茵陈黄连汤大鼠离体肠道渗透机制研究

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  • 国家科技期刊平台
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目的:研究茵陈黄连汤离体肠道渗透机制。方法:采用Ussing chamber技术，应用高效液相色谱-串联质谱法检测茵陈黄连汤大鼠经离体肠道渗透后有效成分阿魏酸的质量浓度，研究不同肠段阿魏酸的吸收参数。结果:阿魏酸在空肠转运的累积透过量(Qtn)、透过百分率与表观渗透系数(Papp)均显著大于在回肠与结肠中的数据(P＜0。05)，在回肠与结肠之间的Qtn、透过百分率、Papp比较差异无统计学意义;阿魏酸在各段小肠的吸收高峰均出现在80 min左右。结论:茵陈黄连汤经肠道透析后的阿魏酸成分在大鼠体外空肠、回肠、结肠中均有吸收，主要吸收部位为空肠段。
Study on in vitro Intestinal Permeability Mechanism of Yinchen Huanglian Decoction
OBJECTIVE:To study the in vitro intestinal permeability mechanism of Yinchen huanglian decoction.METHODS:Ussing chamber and HPLC-MS/MS method was used to detect the concentration of ferulaic acid in Yinchen huanglian decoction after penetrating through isolated intestinal tract; the absorption parameters of ferulaic acid in different intestinal segments were studied.RESULTS:The accumulated absorption amount (Q),absorption percentage and Pav of ferulic acid in jejunum were significantly higher than in ileum and colon(P＜0.05),but there was no significant difference between ileum and colon.The maximal absorption of ferulic acid in small intestines all appeared at about 80 min.CONCLUSIONS:Femlic acid in Yinchen huanglian decoction could be absorbed by jejunum,ileum and colon of rats in vitro,and mainly absorbed by jejunum.

Yinchen huanglian decoctionFerulic acidPharmacokineticsHPLC-MS/MSUssing chamber method

林丹、郑萍、李国锋

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南方医科大学南方医院药学部,广州510515

茵陈黄连汤 阿魏酸 药动学 高效液相色谱-串联质谱法 Ussing chamber技术

广东省医学科研基金立项课题广东省医学科学研究基金

A2010361WSTJJ20091215440111197303218823

2013

10.6039/j.issn.1001-0408.2013.39.03

中国药房
中国医院协会,中国药房杂志社

中国药房

CSTPCDCSCD
影响因子：0.956
ISSN：1001-0408
年,卷(期)：2013.24(39)
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