Study on HPLC Fingerprints of Lonicera macranthoides from Chongqing Xiushan Area
OBJECTIVE:To establish the fingerprint of Lonicera macranthoides from Chongqing Xiushan area.METHODS:HPLC method was adopted.The separation was performed on Phenomenex Synergi Hydro-RP 80A C18 (250 mm × 4.6 mm,4 μm)with mobile phase consisted of acetonitril-0.5％ phosphoric acid(gradient elution) at the flow rate of 1 ml/min.The column temperature was 35 ℃,and detective wavelengh was set at 327 nm.The similarity of samples was evaluated by using TCM chromatographic Fingerprint Similarity Evaluatio System(2004 A edition).RESULTS:HPLC fingerprints of L.macranthoides from 28 producing areas of Chongqing Xiushan area was established with good similarity(＞0.96) and separation; 8 peaks in the chromatogram were common,which was in line with the requirements of TCM chromatographic fingerprints.CONCLUSIONS:The method is accurate and reliable,and can provide scientific basis for the quality control of L.macranthoides.
Lonicera macranthoidesHPLCFingerprintChongqing Xiushan area
国家科技期刊平台
NSTL
万方数据
维普
