Study on the Fingerprint of Artemisia argyi by HPLC
OBJECTIVE:To establish the HPLC fingerprint ofArtemisia argyi.METHODS:HPLC was conducted with ZORB-AX Extend-C18 column; the mobile phase was acetonitrile-0.2％ phosphoric acid solution (gradient elution) ; detection wavelength was 330 nm and flow rate was 1.0 ml/min with column temperature of 30 ℃.The fmgerprint of 10 batches ofA.argyi was determined with the reference substance of naringin and Chromatographic Fingerprint Similarity Evaluation Systen was used for similarity evaluation.RESULTS:There were 12 common peaks in the 10 batches ofA.argyi samples,and 3 common peaks were identified,including chlorogenic acid,naringin and kaempferol.The similarity of the 10 batches of samples was more than 0.90.CONCLUSIONS:The established fmgerprint can be used for the evaluation and quality control ofA.argyi.
国家科技期刊平台
NSTL
万方数据
维普
