首页|单针藻的化学成分及其体外抗菌、抗氧化活性研究

单针藻的化学成分及其体外抗菌、抗氧化活性研究

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  • 国家科技期刊平台
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  • NSTL
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目的：研究单针藻的化学成分，并对其中分离出的化合物进行体外抗菌、抗氧化活性考察。方法：对单针藻乙醇提取物经石油醚、乙酸乙酯和正丁醇萃取，利用硅胶柱色谱、高效液相色谱、气相色谱-质谱法对单针藻乙酸乙酯部位萃取物进行分离和化学成分分析，并根据理化性质和核磁共振谱等分析鉴定化合物的结构；对分离出的4种化合物分别采用刃天青法测定其对铜绿假单胞菌、白色念珠菌、枯草芽孢杆菌、大肠埃希菌的最低抑菌浓度（MIC），1，1-二苯基-2-苦基-肼基（DPPH）法测定自由基清除率[以半数清除浓度（IC50）计]、亚铁还原能力法（以FRAP值计）测定总还原能力。结果：从单针藻乙酸乙酯萃取部位的E4、E5段分离得到化合物1～6，分别被鉴定为豆甾醇、邻苯二甲酸二丙酯、3-吲哚甲酸、黑麦草素、黑麦草内脂、5-羟基-3，4-二甲基-5-戊基-2（5H）-呋喃酮；化合物3～6的MIC在10～500μg/mL内，IC50在22.02～71.01μg/mL内，FRAP值在（62.04±5.36）～（281.22±8.3）μmol/L内。结论：单针藻含有多种脂类和烷酸类物质，具有一定的体外抗菌、抗氧化活性。
Study on Chemical Compounds and in vitro Antibacterial and Antioxidant Activities of Monoraphidium dy-bowskii
OBJECTIVE:To study chemical compounds of Monoraphidium dybowskii,and to investigate the in vitro antibacte-rial and antioxidant activities of isolated compounds. METHODS:The ethanol extract of M. dybowskii were extracted with aether petrolei,ethyl acetate and n-butyl alcohol. The ethyl acetate extract was separated from M. dybowskii and chemical components were analyzed by sillica gel column chromatogram,HPLC and GC-MS. Their structures were identified according to physicochemi-cal properties and NMR. MIC of 4 isolated compounds to Pseudomonas aeruginosa,Candida albicans,Bacillus subtilis and Esche-richia coli were determined by resazurin disc test. Free radical scavenging rate(concluated by IC50)and reducing capacity were mea-sured by 1,1-diphenyl-2-picryl-diazanyl (DPPH) and ferric reducing antioxidant power (FRAP) assay. RESULTS:Compounds 1-6 were obtained from E4 and E5 segments of ethyl acetate extract of M. dybowskii,and their structures were identified as stigmas-terol,diisonoyladipate,indole-3-carboxylic acid,(+)-epiloliolide,(-)-loliolide,5-hydroxy-3,4-dimethy-5-pentyl-2(5H)-furanone. MIC of compounds 3-6 were 10-500 μg/mL,and IC50 ranged 22.02-71.01 μg/mL;FRAP ranged (62.04 ± 5.36)-(281.22 ± 8.3) μmol/L. CONCLUSIONS:M. dybowskii contains multiple lipid and alkanoic acid,and possesses certain in vitro antibacterial and antioxidant activities.

Monoraphidium dybowskiiEthyl acetateExtractChemical componentsIdentificationAntibacterialAntioxi-dant

赵震宇、罗宁、陈晨、李昂、马莎莎、刘积光、王孟、刘平怀

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海南大学材料与化工学院/海南大学热带作物种质资源保护与开发利用教育部重点实验室，海口 570228

单针藻 乙酸乙酯部位 萃取物 化学成分 鉴定 抗菌 抗氧化

国家科技支撑计划项目国家科技型中小企业技术创新基金海南省中药现代化科技专项海南省产学研一体化项目

2011BAD14B0113C26244604892ZY201327CXY20150034

2017

10.6039/j.issn.1001-0408.2017.04.09

中国药房
中国医院协会,中国药房杂志社

中国药房

CSTPCD北大核心
影响因子：0.956
ISSN：1001-0408
年,卷(期)：2017.28(4)
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