首页|苗药朱砂根的HPLC指纹图谱研究

苗药朱砂根的HPLC指纹图谱研究

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原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
目的:建立苗药朱砂根的高效液相色谱(HPLC)指纹图谱.方法:采用HPLC法.色谱柱为DiamonsilC18,流动相为甲醇-水(梯度洗脱),流速为1.0 mL/min,检测波长为220 nm,柱温为30℃,进样量为10 μL.以11-O-(3;4;5'-三羟基没食子酰基)-岩白菜素为参照,测定16批药材样品的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2012版)进行共有峰指认和相似度评价,并就指纹图谱进行聚类分析.结果:16批朱砂根药材的HPLC图谱有6个共有峰,其中8批药材样品的相似度均＞0.9;16批朱砂根药材可聚为4类.结论:该研究所建指纹图谱可为朱砂根药材的鉴别和质量评价提供参考.
Study on HPLC Fingerprint of Miao Medicine Ardisia crenata
OBJECTIVE:To establish HPLC fingerprints of Miao medicine Ardisia crenata.METHODS:HPLC method was adopted.The determination was performed on Diamonsil C18 column with mobile phase consiste of methanol-water (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was 220 nm,and column temperature was maintained at 30 ℃.The sample size was 10 μL.Using 11-O-(3',4',5'-three-o-galloylhyperin)-bergeninum as reference,HPLC fingerprints of 16 batches of samples were determined.Common identification and similarity evaluation were performed by using TCM Chromatographic Fingerprint Similarity Evaluation Software (2012 edition).Cluster analysis of fmgerprrints was conducted.RESULTS:There were 6 common peaks in HPLC fingerprints of 16 batches of samples.The similarity among 8 batches was more than 0.9.The 16 batches of samples could be clustered into 4 categories.CONCLUSIONS:Established fingerprints can provide reference for identification and quality evaluation ofA.crenata.

Ardisia crenataHPLCFingerprintCluster analysis

孙绪、姚成芬、付思红、巩仔鹏、刘亭、杨畅、查俊、李勇军

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贵州省药物制剂重点实验室,贵阳550004

贵州医科大学药学院,贵阳 550004

民族药与中药开发应用教育部工程研究中心/国家苗药工程技术研究中心,贵阳550004

朱砂根 高效液相色谱法 指纹图谱 聚类分析

贵州省科技计划项目贵州省科技计划项目贵州省教育厅项目贵州省教育厅项目贵州省教育厅项目贵州省优秀青年科技人才培养对象专项

黔科合平台人才[2016]56135677黔教合协同创新字[2013]04黔科合KY字[2013]122黔教研合ZYRC字[2014]012黔科合人字[2015]11号

2017

10.6039/j.issn.1001-0408.2017.30.29

中国药房
中国医院协会,中国药房杂志社

中国药房

CSTPCD北大核心
影响因子：0.956
ISSN：1001-0408
年,卷(期)：2017.28(30)
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