止得咳颗粒在大鼠体内的代谢产物研究
Study on metabolites derived from Zhideke granules in rats in vivo
梁洁 1郑飘雪 2陈辉华 2黄春燕 3梁炎丽 2陆春莲 2谢晶晶 2马玉明 2彭嘉文 2赵立春 4陈日兰5
作者信息
- 1. 广西中医药大学药学院,南宁 530200;广西高校中药提取纯化与质量分析重点实验室,南宁 530200;中药固体制剂制造技术国家工程研究中心华南分中心,南宁 530200;广西壮瑶药工程技术研究中心,南宁 530200
- 2. 广西中医药大学药学院,南宁 530200;广西高校中药提取纯化与质量分析重点实验室,南宁 530200
- 3. 钦州市检验检测中心,广西 钦州 535000
- 4. 广西壮瑶药工程技术研究中心,南宁 530200
- 5. 广西中医药大学附属瑞康医院药剂科,南宁 530011
- 折叠
摘要
目的 分析止得咳颗粒在大鼠体内的代谢产物并推测其代谢途径.方法 将雄性SD大鼠随机分为空白组、给药组(止得咳颗粒,9.45 g/kg),每组灌胃超纯水或相应药液,每天2次,每次灌胃间隔6～8 h,连续3 d.收集各组大鼠血清、粪便、尿液样品,利用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱(UPLC-Q-Exactive-MS)技术鉴定大鼠灌胃止得咳颗粒后上述生物样品中的代谢产物,并推测其代谢途径.结果 大鼠灌胃止得咳颗粒后,在其血清、尿液、粪便样品中共鉴定出16个原型成分(如野鸢尾黄素、黄芩素、绿原酸等)和11个代谢产物(如山柰酚或木犀草素水合产物、绿原酸甲基化产物、黄芩苷羟基化产物).其中,血清样品中鉴定出8个原型成分和4个代谢产物;尿液样品中鉴定出10个原型成分和7个代谢产物;粪便样品中鉴定出8个原型成分和5个代谢产物.结论 止得咳颗粒在大鼠体内的代谢成分主要包括黄芩苷、野鸢尾黄素、绿原酸,主要涉及甲基化、羟基化、葡萄糖醛酸化等代谢途径.
Abstract
OBJECTIVE To analyze the metabolites of Zhideke granules and speculate its metabolic pathway in rats in vivo.METHODS Male SD rats were randomly divided into blank group and administration group(Zhideke granules,9.45 g/kg);they were given ultrapure water or relevant medicine,twice a day,every 6-8 h,for 3 consecutive days.Serum,urine and feces samples of rats were collected,and their metabolites were identified by UPLC-Q-Exactive-MS technique after intragastric administration of Zhideke granules;their metabolic pathways were speculated.RESULTS After intragastric administration of Zhideke granules,16 prototype components(i.g.irisflorentin,baicalin,chlorogenic acid)and 11 metabolites(i.g.hydration products of kaempferol or luteolin,methylation products of chlorogenic acid,and hydroxylation products of baicalin)were identified in serum,urine and feces of rats.Among them,8 prototype components and 4 metabolites were identified in serum samples;10 prototype components and 7 metabolites were identified in urine samples;8 prototype components and 5 metabolites were identified in the fecal samples.CONCLUSIONS The metabolites of Zhideke granules in rats mainly include baicalin,irisflorentin,chlorogenic acid,and the main metabolic pathways included methylation,hydroxylation,glucuronidation.
关键词
止得咳颗粒/超高效液相色谱-四极杆-静电场轨道阱高分辨质谱技术/代谢产物/代谢途径/大鼠Key words
Zhideke granules/UPLC-Q-Exactive-MS/metabolites/metabolic pathway/rat引用本文复制引用
基金项目
广西壮族自治区创新驱动发展专项(科技重大专项)(桂科AA17202046)
广西壮族自治区民族药资源与应用工程研究中心项目(桂发改高技函[2020]2605号)
广西中医药重点学科项目(GZXK-Z-20-64)
广西壮族自治区高等学校千名中青年骨干教师培育计划(桂教人[2019]5号)
广西中医药大学"歧黄工程"高层次人才团队培育项目(第三批)()
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据