首页|黄芩汤的抗须癣毛癣菌活性及作用机制研究

黄芩汤的抗须癣毛癣菌活性及作用机制研究

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研究黄芩汤(HQD)的抗须癣毛癣菌活性及作用机制。方法 通过测定最小抑菌浓度(MIC)、最小杀菌浓度(MFC)、菌丝长度、孢子萌发率、生物量和观察菌丝超微结构评价HQD的抗须癣毛癣菌活性;通过山梨醇保护实验检测HQD对须癣毛癣菌细胞壁的影响;通过测定麦角固醇含量和角鲨烯环氧酶(SE)、羊毛甾醇14α-去甲基化酶(CYP51)的活性考察HQD对须癣毛癣菌细胞膜的影响;通过测定线粒体中苹果酸脱氢酶(MDH)、琥珀酸脱氢酶(SDH)及ATP酶(包括钠钾ATP酶、钙镁ATP酶和总ATP酶)的活性考察HQD对须癣毛癣菌线粒体的影响。结果 HQD对须癣毛癣菌具有显著的抑菌活性，MIC、MFC值分别为3。13、25 mg/mL。经HQD干预后，须癣毛癣菌菌丝长度均显著缩短(P＜0。05);孢子萌发率、生物量、细胞膜中麦角固醇含量和SE、CYP51活性以及线粒体中MDH、SDH、各种ATP酶的活性均显著降低(P＜0。05);细胞结构受到了一定程度的破坏，但细胞壁的完整性没有受影响。结论 HQD具有显著的抗须癣毛癣菌活性，其作用机制与降低细胞膜中麦角固醇含量和SE、CYP51活性以及线粒体相关酶活性有关。
Study on the antifungal activity and mechanism of Huangqin decoction against Trichophyton mentagrophytes
OBJECTIVE To study the antifungal activity of Huangqin decoction(HQD)against Trichophyton mentagrophytes and explore its mechanism.METHODS Minimal inhibitory concentration(MIC),minimal fungicidal concentration(MFC),mycelial length,spore germination rate,biomass and mycelium ultrastructure observation were performed to evaluate the antifungal activity of HQD against T.mentagrophytes.The effects of HQD on the cell wall of T.mentagrophytes were detected through sorbitol protection experiment.By measuring the content of ergosterol and the activities of squalene epoxide(SE)and lanosterol 14α-demethylase(CYP51),the activity of HQD on the cell membrane of T.mentagrophytes was investigated.The effects of HQD on T.mentagrophytes mitochondria were investigated by determining the activities of malate dehydrogenase(MDH),succinate dehydrogenase(SDH),and ATPases(including sodium potassium ATPase,calcium magnesium ATPase,and total ATPase).RESULTS HQD exhibited significant antifungal activity against T.mentagrophytes with MIC of 3.13 mg/mL and MFC of 25 mg/mL.After intervention with HQD,the mycelial length of T.mentagrophytes was significantly shortened(P＜0.05);spore germination rate,biomass,the content of ergosterol in the cell membrane,the activities of SE and CYP51 in the cell membrane and MDH,SDH and ATPase in mitochondria were all decreased significantly(P＜0.05);cell structure had been damaged to a certain extent,but the integrity of the cell wall had not been affected.CONCLUSIONS HQD shows significant antifungal activity against T.mentagrophytes,the mechanism of which may be associated with reducing the content of ergosterol in the cell membrane and the activities of SE,CYP51,and mitochondria-related enzymes.

Huangqin decoctionTrichophyton mentagrophytesantifungal activitymechanism of actioncell membranemitochondria

沈成英、罗忠、章佩、邓冯沂、申宝德、胡建新

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江西省人民医院(南昌医学院第一附属医院)药学部,南昌 330006

南昌大学药学院,南昌 330006

江西省人民医院(南昌医学院第一附属医院)临床医学研究所,南昌 330006

江西中医药大学现代中药制剂教育部重点实验室,南昌 330004

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黄芩汤 须癣毛癣菌 抗真菌活性 作用机制 细胞膜 线粒体

江西省自然科学基金项目江西省卫生健康委科技计划项目江西省中医药中青年骨干人才(第四批)培养计划江西省中医药管理局中药制剂临床安全监测和转化应用重点研究室项目

20232BAB216124202310011赣中医药科教字[2022]7号

2024

10.6039/j.issn.1001-0408.2024.03.08

中国药房
中国医院协会,中国药房杂志社

中国药房

CSTPCD北大核心
影响因子：0.956
ISSN：1001-0408
年,卷(期)：2024.35(3)
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