摘要
目的 分析红凉伞提取物化学成分及入血成分,初步阐明其可能的药效物质基础.方法 采用配对比较法将12只大鼠随机分为空白组和红凉伞给药组,每组6只.每天上、下午各给药1次,连续3 d;第4天再次给药后,取血后制备血清样品.采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱技术对红凉伞提取物和血清样品中化学成分进行分析鉴定.使用Compound Discoverer 3.0软件进行保留时间矫正、峰识别、峰提取等工作,根据二级质谱信息,利用Thermo mzCloud在线数据库、Thermo mzVault本地数据库,并参照有关文献和对照品质谱信息,初步鉴定红凉伞化学成分及入血成分.结果 从红凉伞提取物中共鉴定出34个化合物,主要为香豆素类、黄酮类、有机酸类、氨基酸类等成分,包括岩白菜素、槲皮素、没食子酸、L-焦谷氨酸等化合物;从血清样本中共鉴定出5个入血成分,分别为L-焦谷氨酸、丁香酸、岩白菜素、朱砂根皂苷A、麦考酚酸.结论 本研究初步推测L-焦谷氨酸、丁香酸、岩白菜素、朱砂根皂苷A、麦考酚酸是红凉伞的药效物质基础.
Abstract
OBJECTIVE To analyze the chemical constituents and components absorbed into plasma of the extract of Ardisia crenata and to elucidate its possible pharmacodynamic material basis.METHODS Overall,12 rats were randomly assigned to the blank group(n=6)and A.crenata group(n=6)by the paired comparison method.The drug was administered once daily in the morning and afternoon for three days.Serum samples were prepared from serum after redosing on 4th day.The UPLC-QE-HF-MS/MS was used to analyze and identify the chemical constituents in A.crenata extract and serum samples.Compound Discoverer 3.0 was employed for retention time correction,peak identification,and peak extraction.According to the secondary mass spectrometry information,the Thermo mzCloud online and Thermo mzVault local databases,referring to the relevant literature and control quality spectrum information were used to preliminarily identify the chemical constituents and components absorbed into plasma of A.crenata.RESULTS A total of 34 compounds were identified from the extract of A.crenata,mainly coumarins,flavonoids,organic acids,amino acids,including bergenin,quercetin,gallic acid,L-pyroglutamic acid,etc.Besides,5 components absorbed into plasma were identified from serum samples:L-pyroglutamic acid,syringic acid,bergenin,cinnabar root saponin A,and mycophenolic acid.CONCLUSIONS L-pyroglutamic acid,syringic acid,bergenin,cinnabar root saponin A,and mycophenolic acid may act as the pharmacodynamic material basis of A.crenata.
基金项目
贵州省科技计划项目(黔科合基础-ZK[2022]一般483)
贵州省科技成果应用及产业化(非临床专项)项目(黔科合成果[2021]一般137)
贵州省教育厅高校科技创新团队(黔教技[2023]069号)
贵州省教育厅高等学校科学研究项目(青年项目)(黔教技[2022]216号)
安顺市科技计划项目(安市科社[2022]15号)
贵州中医药大学博士启动基金(贵中医博士启动[2019]16)
维普
万方数据