Establishment of UPLC-MS/MS method for the determination of plasma concentration of three common carbapenem antibiotics
OBJECTIVE To establish a UPLC-MS/MS method for the determination of plasma concentration of three carbapenem antibiotics,i.e.ertapenem(ETP),imipenem(IPM)and meropenem(MEM).METHODS After protein precipitation with methanol,the plasma samples were separated by ACQUITY UPLC BEH C18 column(2.1 mm×50 mm,1.7 μm)using stable isotopes of three antibiotics(ETP-D4,IPM-D4,MEM-D6)as the internal standard.The mobile phases were 98%acetonitrile +2%water +0.1%formic acid and 98%water +2%acetonitrile +0.1%formic acid,by gradient elution.The flow rate was 0.3 mL/min and the column temperature was 40℃.Scanning analysis was performed in the positive ion and multiple reaction monitoring mode.RESULTS The method had good specificity,good linearity(r2≥0.993)in the range of 0.2-200,0.1-100 and 0.1-100 μg/mL of ETP,IPM and MEM,and good intra-batch and inter-batch precision and accuracy(all RE≤5.14%,all RSD≤11.15%),the matrix effect and extraction recovery were consistent(RSD≤12.99%).CONCLUSIONS This study establishes the UPLC-MS/MS method to simultaneously quantify the plasma concentration of ETP,IPM and MEM.The method has the advantages of simple pretreatment,short detection time and small sample quantity to meet clinical requirement.
万方数据
维普
