Establishment of a method for detecting propofol concentration in plasma and its application in patients with lymphedema
OBJECTIVE To establish a method for the determination of propofol concentration in human plasma and apply it in patients with lymphedema.METHODS The concentration of propofol was determined by UPLC-MS/MS after protein precipitation of plasma samples using thymol as internal standard.The sample was eluted on a Kinetex C18 column with a mobile phase consisting of acetonitrile(A)-water(B)for gradient elution at the flow rate of 200 μL/min.The sample size was 5 μL,and the column temperature was set at 40℃.The sample chamber temperature was 15℃.Using multi-reaction monitoring mode,the ion pairs for quantitative analysis were m/z 177.0→161.2(propofol)and m/z 149.0→133.1(internal standard),respectively.The above method was used to determine the plasma concentration of propofol in 6 patients with lymphedema.RESULTS The linear range of propofol was 50-5 000 ng/mL(r=0.995 0).RSDs of within-and between-batch precision were not more than 8.08%;no endogenous interference,carryover effect,or dilution effect was observed in blank plasma.The extraction recovery ranged from 89.80%to 93.73%,and matrix effects were within the range of 97.93%-101.73%.RSDs of the stability test were all lower than 3.27%.During intraoperative TCI 2-30 min,the plasma concentration of propofol in 6 patients was maintained in the range of 1 865.3-6 056.2 ng/mL,and the propofol was almost excreted within 4-8 h after operation.CONCLUSIONS The established UPLC-MS/MS method in this study can achieve the determination of propofol and a simple and fast sample pretreatment process without derivatization;it is proved to be suitable for the concentration monitoring of propofol in plasma samples of patients with lymphedema.
万方数据
维普
