Ameliorative effect and mechanism of Sanwei ganlu on hepatic fibrosis in rats
OBJECTIVE To investigate the ameliorative effects and mechanism of Sanwei ganlu on hepatic fibrosis in rats.METHODS The rats were randomly divided into normal group,model group,silibinin group(positive control,50 mg/kg),and Sanwei ganlu low-dose,medium-dose,and high-dose groups(80,250,800 mg/kg).Except for normal group,hepatic fibrosis rat models were established by intraperitoneal injection of CCl4 in the other groups of rats.Starting from the 6th week of modeling administration,they were given normal saline or corresponding drugs intragastrically at the same time.At the end of the ninth-week experiment,liver and spleen indexes of rats were calculated;the pathological structure and fibrosis changes of liver tissue were observed by HE,Masson and Sirus Red staining.The contents of alanine transaminase(ALT),aspartate transaminase(AST),procollagen type Ⅲ(PC Ⅲ),collagen type Ⅳ(COL-Ⅳ),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α)and IL-1β in serum,and hyaluronic acid(HA)and laminin(LN)in liver tissue were all detected.RESULTS Compared with the model group,the liver injury and collagen fiber deposition of rats were improved to different extents in Sanwei ganlu groups and silibinin group;the contents of ALT,AST,PC Ⅲ,COL-Ⅳ,IL-6,TNF-α and IL-1β in serum as well as the contents of HA and LN in liver tissue significantly decreased(P＜0.05 or P＜0.01).CONCLUSIONS Sanwei ganlu can alleviate the progression of hepatic fibrosis in rats,possibly by inhibiting the synthesis of collagen fiber,reducing transaminase content,down-regulating the levels of HA,LN,PC Ⅲ and COL-Ⅳ,and reducing the inflammatory response.
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