OBJECTIVE To investigate the effects of aucubin(Auc)on the malignant biological behavior of breast cancer cells by regulating cyclin-dependent kinase 1(CDK1)/cyclin B1(CCNB1)/Polo-like kinase 1(PLK1)signaling pathway.METHODS Human breast cancer cells MCF-7 were divided into control group,Auc low-,medium-and high-concentration groups(AUC-L,AUC-M,AUC-H groups,20,40 and 80 μmol/L Auc),Auc-H+pcDNA-NC group(80 μmol/L Auc+transfected pcDNA-NC plasmid),and Auc-H+pcDNA-CDK1 group(80 μmol/L Auc+transfected pcDNA-CDK1 plasmid).Cell proliferation,clonal formation,invasion and migration abilities,apoptosis and cycle distribution,and the expressions of related proteins of apoptosis,epithelial-mesenchymal transformation(EMT)and CDK1/CCNB1/PLK1 signaling pathway were detected in each group.The transplanted tumor model of BALB/c nude mice was established by subcutaneous inoculation of MCF-7 cell suspension,and the mice were divided into control group and Auc group(12 mice in each group).The tumor volume,mass and the expressions of related proteins of CDK1/CCNB1/PLK1 signaling pathway in tumor tissues were detected.RESULTS Compared with control group,the number of clonal formation,proliferation rate,cell invasion number,scar healing rate,G1/G0 phase and S phase cell proportions,and the expressions of B cell lymphoma-2(Bcl-2),N-cadherin,fibronectin,CDK1,CCNB1 and PLK1 were decreased significantly(P＜0.05).The apoptotic rate,G2/M phase cell proportion and the expressions of Bcl-2 associated X protein and E-cadherin were significantly increased,in a dose-dependent manner(P＜0.05).Compared with the Auc-H+pcDNA-NC group,there was no statistical significance in the above indexes in the Auc-H group(P＞0.05),while the above indexes in the Auc-H+ pcDNA-CDK1 group were significantly reversed(P＜0.05).Compared with the control group,the tumor volume and mass,and the expressions of CDK1,CCNB1 and PLK1 in tumor tissue of Auc group were significantly decreased(P＜0.05).CONCLUSIONS Auc can inhibit the proliferation,invasion and migration of breast cancer cells,induce cell cycle arrest,and inhibit the progression of EMT,which may be related to inhibiting the activation of the CDK1/CCNB1/PLK1 signaling pathway.
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