Establishment of HPLC fingerprint of Shuangdong capsules and the spectral effect relationship of its anti-inflammatory effect
OBJECTIVE To establish HPLC fingerprint of Shuangdong capsules,and to study the spectral effect relationship of its anti-inflammatory effect.METHODS The fingerprints of 15 batches of Shuangdong capsules were established by HPLC,and the similarity evaluation was carried out;the foot swelling model was established to investigate the anti-inflammatory activity of Shuangdong capsules.The gray correlation analysis method was used to construct the spectral effect relationship for the anti-inflammatory effect of Shuangdong capsules using the swelling rate of rat foot and the levels of malondialdehyde(MDA),superoxide dismutase(SOD),prostaglandin E2(PGE2),interleukin-6(IL-6),IL-8,IL-1β and tumor necrosis factor-α(TNF-α)in right hindfoot tissues as the pharmacodynamic indexes of anti-inflammatory effects.RESULTS Overall 15 batches of Shuangdong capsules identified 20 common peaks,the similarities were all greater than 0.97,and a total of 8 chromatographic peaks were identified.According to the gray correlation analysis,the correlation degrees between the peak area and the foot swelling rate and the levels of MDA,SOD,PGE2,IL-6,IL-8,IL-1β and TNF-α in 15 batches of Shuangdong capsules were 0.621 1-0.783 5,0.564 3-0.827 9,0.581 0-0.845 3,0.564 9-0.855 0,0.583 1-0.856 4,0.576 5-0.863 5,0.564 1-0.838 0 and 0.572 5-0.851 3,respectively.Among them,the chemical components represented by peak 4(geniposidic acid),peak 10(chlorogenic acid)and the chemical composition represented by peak 2 were strongly correlated with anti-inflammatory efficacy indicators.CONCLUSIONS In this study,HPLC fingerprints of 15 batches of Shuangdong capsules were successfully established.Among them,geniposidic acid,chlorogenic acid may be its anti-inflammatory ingredients.
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