益气活血方对大鼠腰椎间盘突出的影响及机制
Effects and mechanism of Yiqi huoxue decoction on lumbar disc herniation in rats
白雪 1孙孝先 2郭杨 2马勇 1刘孟敏 3姜宏 4林顺 4曹瑞 5袁永丰 6刘锦涛4
作者信息
- 1. 南京中医药大学中西医结合学院,南京 210023;南京中医药大学骨伤修复与重建新技术实验室,南京 210023
- 2. 南京中医药大学骨伤修复与重建新技术实验室,南京 210023
- 3. 南京中医药大学中西医结合学院,南京 210023
- 4. 南京中医药大学附属苏州市中医院骨伤科,江苏 苏州 215008
- 5. 泰州市姜堰中医院脊柱骨伤科,江苏 泰州 225300
- 6. 南京中医药大学附属医院放射科,南京 210023
- 折叠
摘要
目的 探讨益气活血方(YQHX)对大鼠腰椎间盘突出的影响及机制.方法 大鼠随机分为假手术组、模型组、核因子κB(NF-κB)抑制剂组(QNZ组,1 mg/kg)、YQHX组(9.1 g/kg)及联合组(YQHX+QNZ组,剂量同各单药组),每组10只.除假手术组外,其余各组大鼠建立腰椎间盘突出症模型,各给药组大鼠腹腔注射QNZ和/或灌胃YQHX,每天给药1次,连续8周.评估各组大鼠椎间盘突出严重程度,观察椎间盘组织形态学变化、髓核细胞自噬变化,检测血清中肿瘤坏死因子α(TNF-α)水平、椎间盘组织中B细胞淋巴瘤2/腺病毒E1B相互作用蛋白3(BNIP3)、苄氯素1(Beclin-1)阳性细胞百分比,核因子κB(NF-κB)p65磷酸化水平,肿瘤坏死因子受体相关因子2(TRAF-2)、TRAF-3、BNIP3、微管相关蛋白轻链3B(LC3B)蛋白表达水平,以及NF-κB p65、LC3B、p62、BNIP3及Beclin-1 mRNA表达水平.结果 与模型组比较,YQHX组大鼠椎间盘Pfirrmann分级评分显著降低,病理损伤减轻;髓核细胞自噬小体数量增加;血清中TNF-α水平和椎间盘组织中p62 mRNA表达水平均显著降低;椎间盘组织中BNIP3、Beclin-1阳性细胞百分比,NF-κB p65磷酸化水平,TRAF-2、TRAF-3、BNIP3、LC3B蛋白表达水平,NF-κB p65、LC3B、BNIP3、Beclin-1 mRNA表达水平均显著升高(P＜0.05).加入QNZ可部分逆转YQHX组上述指标的变化.结论 YQHX可能通过激活NF-κB信号通路促进大鼠椎间盘自噬水平升高,减轻炎症反应,延缓腰椎间盘突出症进展.
Abstract
OBJECTIVE To investigate the effects and mechanism of Yiqi huoxue decoction(YQHX)on lumbar disc herniation in rats.METHODS Rats were randomly divided into sham operation group,model group,NF-κB inhibitor group(QNZ group,1 mg/kg),YQHX group(9.1 g/kg)and combination group(YQHX+QNZ group,the same dose as each single drug group),with 10 rats in each group.Except for sham operation group,lumbar disc herniation model of rats was induced in other groups;administration groups were given QNZ intraperitoneally or/and YQHX intragastrically,once a day,for 8 consecutive weeks.The severity of intervertebral disc herniation was evaluated in each group;the pathological changes of intervertebral discs and the changes of autophagy of nucleus pulposus cells were all observed;the level of tumor necrosis factor-α(TNF-α)in serum,and the ratios of Bcl-2/adenovirus E1B interacting protein 3(BNIP3)and Beclin-1 positive cells in intervertebral disc tissues were detected;the phosphorylation of nuclear factor-κB(NF-κB)p65,the expressions of tumor necrosis factor receptor-associated factor-2(TRAF-2),TRAF-3,BNIP3 and LC3B protein,and mRNA expressions of NF-κB p65,LC3B,p62,BNIP3 and Beclin-1 were determined.RESULTS Compared with model group,Pfirrmann grading score decreased significantly,the pathological injury of intervertebral disc tissue was relieved in YQHX group;the number of autophagosomes in nucleus pulposus cells increased;serum level of TNF-α and mRNA expression of p62 in intervertebral disc tissue decreased significantly;the ratios of BNIP3 and Beclin-1 positive cells,the phosphorylation of NF-κB p65,the expressions of TRAF-2,TRAF-3,BNIP3 and LC3B protein as well as the mRNA expressions of NF-κB p65,LC3B,BNIP3 and Beclin-1 decreased significantly in intervertebral disc tissues(P＜0.05).The changes of above indexes in YQHX group were reversed partly in YQHX+QNZ group.CONCLUSIONS YQHX promotes the elevation of autophagy level of intervertebral discs,slows down the inflammatory response and the progression of lumbar disc herniation by activating the NF-κB signaling pathway.
关键词
腰椎间盘突出/炎症反应/NF-κB信号通路/自噬Key words
lumbar disc herniation/inflammatory response/NF-κB signaling pathway/autophagy引用本文复制引用
基金项目
国家自然科学基金面上项目(82074467)
国家自然科学基金面上项目(82374220)
国家中医药局高水平中医药重点学科建设项目(国中医药人教函[2023]85号)
江苏省中医药领军人才培养项目(苏中医科教[2023]17号)
江苏省研究生科研与实践创新计划(KYCX23_2202)
出版年
2024
国家科技期刊平台
NSTL
万方数据