Study on in vitro anti-inflammatory effects and mechanisms of oblongifolins A extracted from Garcinia oblongifolia
OBJECTIVE To investigate the in vitro anti-inflammatory effects and mechanisms of oblongifolins A(OA)extracted from Garcinia oblongifolia.METHODS RAW264.7 cells were used as the research subject and divided into control group(0.5%DMSO),lipopolysaccharide(LPS)group(1 μg/mL),DEX group(10 µmol/L DEX+1 μg/mL LPS),and low-,medium-,and high-concentration groups of OA(7.5,15,30 µmol/L OA+1 μg/mL LPS).Except for the control group,the remaining groups were first stimulated with LPS for 1 hour and then mixed with drugs for 24 hours.The morphological changes of cells were observed in each group.The contents of nitric oxide(NO),reactive oxygen species(ROS),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),IL-1β,IL-4 and IL-10 were detected in cells of each group;mRNA expression levels of TNF-α,IL-6 and IL-1β were measured.The expression of key proteins in the nuclear factor κB(NF-κB)and nuclear factor-erythroid 2-related factor 2(Nrf2)signaling pathways in each group,as well as the nuclear translocation of NF-κB p65 and Nrf2 proteins in control group,LPS group and OA high-concentration group,were detected.RESULTS Compared to the LPS group,the number of spindle-shaped and irregular cells gradually decreased in OA groups,the contents of NO,ROS(except for OA low-concentration group),TNF-α,IL-6 and IL-1β,the mRNA expressions of TNF-α,IL-6(except for OA low-concentration group)and IL-1β as well as the protein expressions of phosphorylated NF-κB p65(p-NF-κB p65),p-IκBα,and Kelch-like ECH-associated protein 1(Keap1)were decreased significantly(P＜0.05).The contents of IL-4 and IL-10,protein expressions of IκBα,Nrf2(except for OA low-and medium-concentration groups),HO-1(except for OA low-concentration group)and NQO1 were all increased significantly(P＜0.05).OA of high concentration could inhibit NF-κB p65 protein nuclear translocation and promote Nrf2 protein nuclear translocation.CONCLUSIONS OA can suppress LPS-induced inflammation in RAW264.7 macrophages.The underlying molecular mechanism likely entails the inhibition of the NF-κB signaling pathway,the activation of the Nrf2 signaling pathway and the reduction of ROS and inflammatory factor release.
国家科技期刊平台
NSTL
万方数据
