首页|酒蒸桑寄生的炮制工艺优化及质量表征研究

酒蒸桑寄生的炮制工艺优化及质量表征研究

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目的 优化桑寄生酒蒸炮制工艺,并对其质量进行表征。方法 以扁蓄苷、槲皮苷、槲皮素含量和外观性状评分为评价指标,采用层次分析法-熵权法确定各指标权重,并以各指标综合评分为响应值,采用Box-Behnken响应面法考察料液比(g/mL)、闷润时间、蒸制时间对酒蒸桑寄生炮制工艺的影响,优化最佳炮制工艺,并进行验证。将15批不同产地桑寄生饮片按最佳炮制工艺制备酒蒸桑寄生,并对其质量进行表征。结果 酒蒸桑寄生最佳炮制工艺为取桑寄生饮片100 g,加入黄酒20 mL密封闷润2 h,常压蒸制1 h,取出,于50℃条件下干燥即得。以最佳炮制工艺制得的酒蒸桑寄生表面呈红褐色或棕褐色,其粉末呈深棕色,质地坚硬或脆,易折断,略有酒香气,味涩;茎横切面显微鉴别和薄层色谱鉴别结果同生桑寄生;水分、总灰分、酸不溶性灰分含量分别为3。92%~8。75%、2。27%~5。08%、0。19%~0。82%,水溶性浸出物含量为11。28%~18。56%,醇溶性浸出物含量为3。36%~8。58%,扁蓄苷、槲皮苷和槲皮素的含量分别为0。22~1。64、0。26~2。45、0。01~0。38 mg/g。结论 本研究成功优化了酒蒸桑寄生的炮制工艺,并对其质量进行了表征,可为该饮片的规范化炮制及质量标准建立提供参考。
Optimization of processing technology and quality characterization of wine-steamed Taxillus chinensis
OBJECTIVE To optimize the steaming and processing technology of wine-steamed Taxillus chinensis,and to characterize its quality.METHODS Using the content of avicularin,quercitrin,quercetin and appearance traits as evaluation indicators,the analytic hierarchy process(AHP)-entropy weight method was used to determine the weights of each indicator,and the comprehensive scores of those indicators were used as response values.Box-Behnken response surface method was used to investigate the effects of solid-liquid ratio(g/mL),soaking time,and steaming time on the processing technology of wine-steamed T.chinensis,optimize the best processing technology,and verify it.Fifteen batches of T.chinensis decoction pieces from different origins were used to prepare wine-steamed T.chinensis using the best processing technology,and their qualities were characterized.RESULTS The optimal processing technology for wine-steamed T.chinensis was to take 100 g of T.chinensis decoction pieces,add 20 mL of yellow wine,seal and moisten for 2 h,steam at normal pressure for 1 h,take out and dry at 50℃.The surface of wine-steamed T.chinensis prepared by the optimal processing technology was reddish brown or brownish,and its powder was dark brown,with a hard or brittle texture that was easy to break,and had a slight aroma of alcohol,and an astringent taste.Results of microscopic and thin-layer identification for the stem cross-section of wine-steamed T.chinensis were the same as those of raw T.chinensis.The contents of moisture,total ash and acid-insoluble ash were 3.92%-8.75%,2.27%-5.08%,and 0.19%-0.82%,respectively;the contents of water-soluble extract were 11.28%-18.56%,and the contents of alcohol-soluble extract were 3.36%-8.58%;the contents of avicularin,quercitrin,and quercetin were 0.22-1.64,0.26-2.45,and 0.01-0.38 mg/g,respectively.CONCLUSIONS This study successfully optimized the processing technology of wine-steamed T.chinensis and preliminarily characterized its quality,which can provide reference for the standardized processing and establishment of quality standards for wine-steamed T.chinensis decoction pieces.

Taxillus chinensiswine-steamedprocessing technologyquality characterizationanalytic hierarchy processentropy weight methodBox-Behnken response surface method

李慧峰、李慧、孟霜、王晓涛、王志伟、孔祥鹏、詹海仙、王颖莉

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山西中医药大学中药与食品工程学院,山西晋中 030619

山西省中西医结合医院中心实验室,太原 030013

桑寄生 酒蒸 炮制工艺 质量表征 层次分析法 熵权法 Box-Behnken响应面法

山西省科技创新人才团队专项山西省中药饮片炮制规范研究项目山西省卫生健康委重点实验室建设项目

20220405100202820220515A2020SYS23

2024

中国药房
中国医院协会,中国药房杂志社

中国药房

CSTPCD北大核心
影响因子:0.956
ISSN:1001-0408
年,卷(期):2024.35(11)
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