首页|鸡骨草水提物灌胃大鼠后的体内成分及代谢途径分析

鸡骨草水提物灌胃大鼠后的体内成分及代谢途径分析

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的 分析鸡骨草水提物灌胃大鼠后的入血和入肝成分,并推测其可能的代谢途径,为鸡骨草的药效物质基础分析提供参考.方法 将雄性SD大鼠随机分为鸡骨草给药组(0.63 g/kg,以生药量计)、空白组,分别单次灌胃相应药液/超纯水,收集各组大鼠的血浆和肝脏样品.采用超高效液相色谱-四极杆-飞行时间质谱(UPLC-Q-TOF/MS)技术对鸡骨草水提物化学成分及入血、入肝成分进行鉴定.结果 从鸡骨草水提物中共鉴定了30个化学成分,主要为生物碱类、黄酮类、有机酸类和环烯醚萜类(如相思子碱、夏佛塔苷和异夏佛塔苷等)等.从血浆样品中鉴定了10个原型成分和9个代谢产物(如原儿茶酸脱羧化硫酸化代谢产物、对羟基苯甲酸还原化硫酸化代谢产物等),从肝脏样品中鉴定了6个原型成分和5个代谢产物(如对羟基苯甲酸硫酸化代谢产物、对羟基苯甲酸脱羧化硫酸化代谢产物等).代谢途径主要包括羟基化、去甲基化、甲基化、硫酸化、葡萄糖醛酸化等.结论 生物碱类、黄酮类、有机酸类是鸡骨草水提物入血和入肝的主要成分,主要涉及羟基化、去甲基化、硫酸化等代谢途径.
Analysis of in vivo components and metabolism pathway in rats after intragastric administration of aqueous extract from Abrus cantoniensis
OBJECTIVE To analyze the chemical components that were the absorbed in blood and liver tissue of rats after intragastric administration of aqueous extract from Abrus cantoniensis,and to speculate its possible metabolic pathways,providing reference for basic analysis of pharmacological substance in A.cantoniensis.METHODS Male SD rats were randomly divided into A.cantoniensis group(0.63 g/kg,calculated by crude drug)and blank group;they were given relevant drug solution/ultrapure water intragastrically.After a single dose,plasma and liver samples of rats in each group were collected.UPLC-Q-TOF/MS technology was used to identify chemical components that were absorbed in the blood and liver tissue of rats.RESULTS Totally,30 chemical constituents were identified from the water extracts of A.cantoniensis,including alkaloids,flavonoids,organic acids,iridoids(such as L-abrine,schaftoside,isoshaftoside).Ten prototype components and nine metabolites(such as decarboxylation and sulfation metabolites of protocatechuic acid,reduced sulfated metabolites of p-hydroxybenzoic acid)were identified from plasma samples;six prototype components and five metabolites(such as sulfated metabolites of p-hydroxybenzoic acid,decarboxylation and sulfation metabolites of p-hydroxybenzoic acid)were identified from liver samples.The main metabolic pathways included hydroxylation,demethylation,methylation,sulfation,glucuronidation,etc.CONCLUSIONS Alkaloids,flavonoids and organic acids are the main components of the aqueous extract from A.cantoniensis that are absorbed into the blood and liver,their metabolism mainly involves hydroxylation,demethylation,and sulfation.

Abrus cantoniensisplasmaliverUPLC-Q-TOF/MSdrug-derived ingredients

陈奎奎、农雨欣、郭忠会、谭勇、黄宏婷、黎金满、韦金锐、韦志英、梁洁

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广西中医药大学药学院,南宁 530200

广西高校中药民族药资源保护与利用重点实验室,南宁 530200

广西高校中药提取纯化与质量分析重点实验室,南宁 530200

鸡骨草 血浆 肝脏 UPLC-Q-TOF/MS 药源性成分

国家自然科学基金区域创新发展联合基金重点支持项目国家自然科学基金项目广西科技基地与人才专项广西自然科学基金项目广西中医药大学第三批"岐黄工程"高层次人才团队培育项目广西中医药大学青年创新团队项目广西中医药大学博士科研启动基金项目广西中医药大学青年基金项目

U23A2052182160771桂科AD212380322022JJB1402862024062018QT0012020BS0152021QN006

2024

10.6039/j.issn.1001-0408.2024.13.06

中国药房
中国医院协会,中国药房杂志社

中国药房

CSTPCD北大核心
影响因子：0.956
ISSN：1001-0408
年,卷(期)：2024.35(13)
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