Screening of effective parts for acute and chronic pain relief of Shaoyao gancao decoction and analysis of its blood components
OBJECTIVE To study the pharmacological substance basis of Shaoyao gancao decoction for relieving acute and chronic pain.METHODS The antispasmodic effect of Shaoyao gancao decoction,ethyl acetate extract of Shaoyao gancao decoction and its effluent part of macroporous resin and 90%ethanol elution part of macroporous resin(the concentration of 4 drugs was 13.44 g/mL according to crude drug)was observed by in vitro small intestine tension test in rats.The acetic acid writhing test was conducted in mice to evaluate the analgesic effects of macroporous resin efflux site and macroporous resin 90%ethanol elution site(the dosage of 2.4 g/kg according to crude drug).The levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),prostaglandin E2(PGE2)and cyclooxygenase-2(COX-2)in serum of mice were detected.The serum prototype and metabolites of mice after intragastric administration of macroporous resin 90%ethanol elution site were identified by high performance liquid chromatogre-time-of-flight mass spectrometry.RESULTS In vitro experiment showed that 90%ethanol eluting part of macroporous resin represented the best antispasmodic effect,and the inhibitory rate of small intestine tension was significantly higher than macroporous resin efflux site of Shaoyao gancao decoction(P＜0.05)without statistical significance,compared with Shaoyao gancao decoction(P＞0.05).In the acetic acid writhing experiment,compared with model group,the writhing times of mice in the macroporous resin 90%ethanol elution part group were reduced significantly(P＜0.05),the writhing latency was prolonged significantly(P＜0.05),and the levels of COX-2,IL-1β,PGE2 and TNF-α in serum were decreased significantly(P＜0.05).Ten kinds of protoproducts including paeoniflorin and glycyrrhizic acid were identified from serum of mice,and twenty-two kinds of metabolites including hydroxylated glycyrrhizin and glucosylated liquiritin were identified.CONCLUSIONS The effective part of Shaoyao gancao decoction for relieving acute and chronic pain is 90%ethanol elution part prepared by macroporous resin from the ethyl acetate extract.Ten components,including glycyrrhetinic acid and paeoniflorin,may be the basis of its pharmacological substances.
Shaoyao gancao decoctionacute and chronic pain reliefserum medicinal chemistrypharmacological substances
万方数据
维普
