Effects of Qiangxin decoction on myocardial mitochondrial homeostasis and energy metabolism in chronic heart failure rats
OBJECTIVE To investigate the effects of Qiangxin decoction on myocardial mitochondrial and energy metabolism in rats with chronic heart failure(CHF)based on mitophagy.METHODS Male SD rats were collected to establish CHF model by ligating the left anterior descending branch of coronary artery.The successful modeling rats were divided into model group,Qiangxin decoction group[12.25 g/(kg·d),calculated by crude drug],captopril group[10.38 mg/(kg·d)],and Qiangxin decoction+captopril group(the same usage and dosage as single drug group)according to a random number table method,with 8 rats in each group.Another 8 normal rats were selected and received threading in the left anterior descending branch of the coronary artery without ligation as the sham operation group.Starting from the second day after successful modeling,the rats in administration groups were given relevant drug intragastrically,twice a day,for consecutive 28 days.After the last medication,the levels of adenosine triphosphate(ATP),adenosine monophosphate(AMP)and free fatty acid(FFA)in infarcted myocardial tissues were detected,the pathological changes and mitochondrial morphology of the infarcted myocardial tissue were observed,as well as the protein expressions of B cell lymphoma-2(Bcl-2),Bcl-2 related X protein(Bax),TANK-binding kinase 1(TBK1),p62 were detected in each group.RESULTS Compared with the sham operation group,the infarcted myocardial tissue fibrosis was changed evidently,with a large number of mitochondrial swelling and fusion,and internal cristae rupture;the levels of AMP and FFA,the protein expressions of Bax and p62 were all increased or up-regulated significantly in infarcted myocardial tissue,while the level of ATP,and the protein expressions of Bcl-2 and TBK1 were all decreased or down-regulated significantly(P＜0.05).Compared with the model group,the pathological changes of infarcted myocardial tissue and mitochondrial swelling had been improved;the levels of AMP and FFA,and the protein expressions of Bax and p62 in infarcted myocardial tissue were significantly decreased or down-regulated in administration groups,while the level of ATP,and the protein expressions of Bcl-2 and TBK1 were increased or up-regulated significantly(P＜0.05).And the effect of Qiangxin decoction+captopril group was better than that of single drug group.CONCLUSIONS Qiangxin decoction can alleviate myocardial fibrosis and mitochondrial swelling in CHF rats,and improve their myocardial energy metabolism,which may be related to regulating the expression of Bcl-2,Bax,TBK1 and p62 proteins and promoting myocardial mitophagy.
万方数据
维普
