Comprehensive evaluation of Yifei qinghua ointment by multi-component quantitative analysis combined with chemometrics and entropy weight TOPSIS
OBJECTIVE To comprehensively evaluate the quality of Yifei qinghua ointment by multi-component quantitative analysis combined with chemometrics and entropy weight-technique for order preference by similarity to ideal solution(TOPSIS)method.METHODS The contents of lobetyolin,syringin,calycosin 7-O-β-D-glucopyranoside,ononin,astraisoflavan-7-O-β-D-glucoside,isomucronulatol 7-O-glucoside,astragaloside Ⅳ,deapi-platycoside E,platycoside E,platycodin D3,feretoside,asperulosidic acid,asperuloside,methylophiopogonanone A and methylophiopogonanone B in 14 batches of Yifei qinghua ointment(S1-S14)were determined by high-performance liquid chromatography method.Then,the quality of 14 batches of Yifei qinghua ointment was analyzed by chemometrics(principal component analysis and orthogonal partial least-squares discriminant analysis)and entropy weight TOPSIS method.RESULTS The results of chemometrics showed that 14 batches of Yifei qinghua ointment could be clustered into three categories,S1-S6 as the first category,S7-S10 as the second category,and S11-S14 as the third category.The values of variable importance for projection of calycosin 7-O-β-D-glucopyranoside,ononin,feretoside,astragalosideⅣ,astraisoflavan-7-O-β-D-glucoside,lobetyolin,methylophiopogonanone A and platycoside E were higher than 1.The results of the entropy weight TOPSIS method showed that the Euclidean closeness of the optimal solution of 14 batches of Yifei qinghua ointment were between 0.152 9 and 0.736 6,and that of sample S14 was the highest(0.736 6).CONCLUSIONS Among 14 batches of Yifei qinghua ointment,sample S14 has the best quality,and 8 components such as calycosin 7-O-β-D-glucopyranoside and ononin may be differential markers affecting the quality of Yifei qinghua ointment.
万方数据
维普
