Effect mechanism of Gualou guizhi granule on neurological function and synaptic plasticity in rats with cerebral ischemia-reperfusion injury
OBJECTIVE To investigate the effect mechanism of Gualou guizhi granule on neurological function and synaptic plasticity in rats with cerebral ischemia-reperfusion injury.METHODS The rats were randomly divided into sham operation group,model group and Gualou guizhi granule group(3.6 g/kg),with 6 rats in each group.The cerebral ischemia-reperfusion injury model was established by the suture occlusion method.Two hours after modeling,the model rats were given relevant medicine or normal saline intragastrically,once a day,for 7 consecutive days.After the last medication,the neurological function of rats was evaluated[calculated by modified neurological severity scores(mNSS)and corner turning percentage];the neuronal apoptosis rate of brain histiocyte in the ischemic side of rats was detected in each group;the positive expressions of growth associated protein 43(GAP43)and microtubule associated protein 2(MAP2)were detected;protein expressions of neuron-specific nuclear protein(NeuN),synaptophysin-1(Syn-1),postsynaptic density protein-95(PSD-95),peroxisome proliferators-activated receptor γ(PPARγ),brain-derived neurotrophic factor(BDNF)and tropomyosin receptor kinase B(TrkB),as well as mRNA expressions of NeuN,Syn-1 and PSD-95 were detected.RESULTS Compared with the model group,mNSS,corner turning percentage and neuronal apoptotic rate were decreased significantly in Gualou guizhi granule group(P＜0.01);GAP43 represented weak immunoreactivity,and MAP2 represented moderate immunoreactivity;protein expressions of NeuN,Syn-1,PSD-95,PPARγ,BDNF,TrkB and mRNA expressions of Syn-1,NeuN,PSD-95 were all increased significantly(P＜0.05 or P＜0.01).CONCLUSIONS Gualou guizhi granule can promote synaptic plasticity by activating BDNF/TrkB signaling pathway,thus playing a protective role in cerebral ischemia-reperfusion injury in rats.
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