Evaluation of antioxidant activity compounds extracted from the pine needle of Cedrus deodara by HPLC-DPPH screening method
OBJECTIVE To evaluate the antioxidant activity compounds extracted from the pine needle of Cedrus deodara.METHODS Using vitamin C(VC)and gallic acid as positive control,HPLC method was employed to determine the changes of the DPPH peak area after antioxidant compounds from pine needle of C.deodara were treated with 1,1-diphenyl-2-picrylhydrazyl radical(DPPH).Then,the antioxidant activities of 3 active parts and 8 monomer compositions from pine needle of C.deodara were evaluated by calculating the inhibition rate of DPPH free radicals and the half inhibitory concentration(IC50).HPLC conditions were using Agilent Eclipse Plus-C18 column(250 mm×4.6 mm,5 μm)with acetonitrile-water(62∶38,V/V)as the mobile phase.The detection wavelength was 517 nm;the flow rate was 1.0 mL/min;the column temperature was 25℃;the injection volume was 20 μL.RESULTS The order of DPPH free radical inhibition activity of active parts from pine needle of C.deodara was total flavonoids＞total polyphenols＞total lignans,with IC50 of 76.10,100.50 and 115.40 μg/mL,respectively.Compared with positive control,except for kaempferol-3-O-β-D-glucoside,the sequence of seven monomer compounds for DPPH free radical inhibition activity was dihydromyricetin＞hypericin＞protocatechuic acid＞gallic acid＞ferulic acid＞myricetin＞kaempferol-3-O-(6″-O-E-feruloyl)-β-D-glucopyranoside＞VC＞isohamnin,with IC50 of 16.50,19.40,23.73,27.24,32.10,32.20,47.60,93.20 and 297.20 μg/mL,respectively.CONCLUSIONS HPLC-DPPH method is established to evaluate the antioxidant activity of pine needle of C.deodara.The pine needle of C.deodara has strong antioxidant activity,among which the effective compounds of total flavonoids,total polyphenols,monomer dihydromyricetin and hypericin are natural antioxidants with development prospects.
pine needle of Cedrus deodaraantioxidant activityeffective fractionchemical constituentHPLC-DPPH
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