Improvement effect and mechanism of Shuhou tongqi formula on intestinal injury in mice with postoperative ileus
OBJECTIVE To study the improvement effect and mechanism of Shuhou tongqi formula on intestinal injury in mice with postoperative ileus(POI).METHODS Mice were randomly divided into sham operation group,model group,positive control group(Mosapride citrate tablets,1.95 mg/kg),and Shuhou tongqi formula group(1.88 g/kg),with 6 mice in each group.Except for the sham operation group,POI model was induced in other groups by typical small intestinal interference.Each group was given relevant drug liquid/water,once a day,for consecutive 2 days.After the last medication,the percentage of carbon powder propulsion in small intestine was detected,and pathomorphological changes in ileum tissue of mice were observed.The serum levels of interleukin-6(IL-6),IL-10,tumor necrosis factor-α(TNF-α),motilin(MTL)and somatostatin(SS)were all detected;the expression levels of Toll-like receptor 4(TLR4),nuclear factor κB(NF-κB p65)and p38 mitogen-activated protein kinase(p38 MAPK)were determined in ileal tissue of mice;the gut microbiota of colon contents was analyzed in each group of mice.RESULTS After the intervention of Shuhou tongqi formula,pathological damage such as intestinal wall atrophy and mucosal capillary congestion in ileum tissue were improved significantly;the percentage of carbon powder propulsion and the serum level of IL-10 and MTL were increased significantly(P＜0.05);however,serum levels of TNF-α,IL-6 and SS,the expressions of TLR4,NF-κB p65 and p38 MAPK were decreased significantly(P＜0.05).The analysis of gut microbiota showed that Shuhou tongqi formula could significantly increase ACE,Chao1,Shannon and PD indexes,and relative abundance of Akkermansia(P＜0.05),but decreased relative abundance of Proteobacteria,Ligilactobacillus and Escherichia-Shigella significantly(P＜0.05).CONCLUSIONS Shuhou tongqi formula can improve intestinal injury and inflammatory reaction of POI mice,the mechanism of which may be associated with inhibiting the activity of TLR4/NF-κB/MAPK signaling pathway,regulating the levels of gastrointestinal hormones and improving the disturbance of intestinal flora in mice.
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