Identification of chemical constituents of modified Yupingfengsan formula
OBJECTIVE To identify the chemical constituents of the modified Yupingfengsan formula.METHODS UPLC-Q-Exactive Orbitrap-MS technology was adopted.The separation was performed on Waters BEH C18 column with acetonitrile(A)-0.1％formic acid solution(B)as mobile phase for gradient elution.The heating electrospray ionization was used for positive and negative ion mode scanning.The scanning range was m/z 50-1 500,and the spray voltage was 2 kV(positive ion mode)and 1.5 kV(negative ion mode).The information of chemical constituents of modified Yupingfengsan formula was collected through literature review to establish a database;the structure of the constituent was identified based on the above database,relevant literature,and chromatography and mass spectrometry information of reference standards.RESULTS & CONCLUSIONS Totally 114 chemical constituents were identified from modified Yupingfengsan formula,including 31 flavonoids,39 phenylpropanoids,5 saponins,8 terpenoids,3 chromones,3 curcuminoids,etc.Based on the comparison of reference standards,8 constituents were ultimately determined,including magnoflorine,calycosin,calycosin glycoside,cimifugin,5-O-methylvisammioside,sec-O-glucosylhamaudol,luteolin and mangiferin.These constituents mainly involved glycosylation cleavage,retro Diels-Alder fragmentation,glycosylation loss,neutral molecule loss and other fragmentation pathways.
万方数据
维普
