Study on protective effect and mechanism of Bupleurum chinense polysaccharides on acute liver injury in mice
OBJECTIVE To study the protective effect and mechanism of Bupleurum chinense polysaccharides(BCP)on acute liver injury(ALI)in mice.METHODS Overall 40 mice were randomly divided into normal group,model group,positive control group(Baogan tablet,550 mg/kg),BCP high-dose and low-dose groups(400,100 mg/kg),with 8 mice in each group.The drug was administered intragastrical once a day for 7 days.One hour after the last administration,except for the normal group,mice in other groups were injected with 20 mg/kg concanavalin A solution through the tail vein to establish ALI model.After injection of concanavalin A solution for 12 h,the liver and spleen indexes of mice were measured,and the pathological changes of liver and spleen tissue were observed;the serum levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT)and lactate dehydrogenase(LDH)were detected,and the levels of superoxide dismutase(SOD)and malondialdehyde(MDA)in liver tissue were detected.The levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and IL-1β in serum and liver tissue of mice were determined,as well as the protein expression levels of nuclear factor-κB(NF-κB),Toll-like receptor 4(TLR4),nuclear factor erythroid 2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)in liver tissue were also detected.RESULTS Compared with the normal group,the liver tissue of mice in the model group was necrotic and infiltrated with inflammatory cells;spleen enlargement,increased bleeding and decreased lymphocytes were observed,liver and spleen indexes were increased significantly(P＜0.01);the serum levels of AST,ALT and LDH,the levels of TNF-α,IL-6 and IL-1β in serum and liver tissue,as well as the MDA level,protein expressions of TLR4,NF-κB and HO-1 in liver tissue were all increased significantly(P＜0.05 or P＜0.01).The levels of SOD and protein expression of Nrf2 in liver tissue were all decreased significantly(P＜0.05).Compared with the model group,the pathological damages of the liver and the spleen tissues in mice alleviated in BCP high-dose and low-dose groups,and most of liver and spleen indexes,the above indexes of serum and liver tissue were reversed significantly(P＜0.05 or P＜0.01).CONCLUSIONS BCP has a protective effect on ALI,the mechanism of which may be related to the inhibition of TLR4/NF-κB signaling pathway and the activation of the Nrf2/HO-1 signaling pathway.
万方数据
维普
