OBJECTIVE To study the protective effect and mechanism of Bupleurum chinense polysaccharides(BCP)on acute liver injury(ALI)in mice.METHODS Overall 40 mice were randomly divided into normal group,model group,positive control group(Baogan tablet,550 mg/kg),BCP high-dose and low-dose groups(400,100 mg/kg),with 8 mice in each group.The drug was administered intragastrical once a day for 7 days.One hour after the last administration,except for the normal group,mice in other groups were injected with 20 mg/kg concanavalin A solution through the tail vein to establish ALI model.After injection of concanavalin A solution for 12 h,the liver and spleen indexes of mice were measured,and the pathological changes of liver and spleen tissue were observed;the serum levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT)and lactate dehydrogenase(LDH)were detected,and the levels of superoxide dismutase(SOD)and malondialdehyde(MDA)in liver tissue were detected.The levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and IL-1β in serum and liver tissue of mice were determined,as well as the protein expression levels of nuclear factor-κB(NF-κB),Toll-like receptor 4(TLR4),nuclear factor erythroid 2-related factor 2(Nrf2)and heme oxygenase-1(HO-1)in liver tissue were also detected.RESULTS Compared with the normal group,the liver tissue of mice in the model group was necrotic and infiltrated with inflammatory cells;spleen enlargement,increased bleeding and decreased lymphocytes were observed,liver and spleen indexes were increased significantly(P＜0.01);the serum levels of AST,ALT and LDH,the levels of TNF-α,IL-6 and IL-1β in serum and liver tissue,as well as the MDA level,protein expressions of TLR4,NF-κB and HO-1 in liver tissue were all increased significantly(P＜0.05 or P＜0.01).The levels of SOD and protein expression of Nrf2 in liver tissue were all decreased significantly(P＜0.05).Compared with the model group,the pathological damages of the liver and the spleen tissues in mice alleviated in BCP high-dose and low-dose groups,and most of liver and spleen indexes,the above indexes of serum and liver tissue were reversed significantly(P＜0.05 or P＜0.01).CONCLUSIONS BCP has a protective effect on ALI,the mechanism of which may be related to the inhibition of TLR4/NF-κB signaling pathway and the activation of the Nrf2/HO-1 signaling pathway.
维普
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