Study on pharmacodynamic substances of anti-inflammatory effect of Zhuang medicine Stahlianthus involucratus based on metabolism in rats
OBJECTIVE To provide reference for basic analysis of the pharmacodynamic substance in Stahlianthus involucratus.METHODS Overall 24 SD male rats were randomly divided into blank group(purified water),and administration group(ethanol extract of S.involucratus,15.75 g/kg,calculated by crude drug),with 12 rats in each group.They were given drug liquid/purified water intragastrically,twice a day,every 6-8 h,for consecutive 3 days.After medication,the blood,urine and fecal samples were collected from two groups of rats.UPLC-Q-Exactive-MS technology was used to identify the chemical constituents in the ethanol extract of S.involucratus,and metabolites in the blood,urine and fecal of rats after intragastrical administration of the ethanol extract of S.involucratus.Multivariate statistical analysis was employed to screen various serum metabolites.Metabolic pathways were analyzed by MetaboAnalyst 5.0 platform.RESULTS A total of 38 chemical constituents were identified from the ethanol extract of S.involucratus,including fourteen prototype components and three metabolites identified from urine samples,nine prototype components identified from fecal samples,and ten prototype components and one metabolite identified from serum samples.A total of 71 differential metabolites were screened from two groups of rat serum samples,of which 44 differential metabolites,such as ferulic acid,glycyrrhizin,were up-regulated and 27 differential metabolites,such as arachidonic acid,phenylacetylglutamine,were down-regulated.The 71 differential metabolites were mainly enriched in 11 metabolic pathways,including phenylalanine metabolism,linoleic acid metabolism,arachidonic acid metabolism,and tryptophan metabolism.CONCLUSIONS Ferulic acid,liquiritigenin,isofraxidin and formononetin may be the material basis that directly exert pharmacological effects of S.involucratus.S.involucratus may exert anti-inflammatory effects by affecting metabolic pathways,including arachidonic acid metabolism and tryptophan metabolism.
万方数据
维普
