Effect of Runzao zhiyang capsule on gut microbiota and short-chain fatty acids in eczema rats
OBJECTIVE To study the effect of Runzao zhiyang capsule(RZZY)on gut microbiota and its metabolites short-chain fatty acids(SCFA)in eczema rats,in order to reveal the improvement effect and possible mechanism of RZZY on eczema.METHODS The rats were divided into normal group,model group,desloratadine group(1.5 mg/kg),and RZZY low-dose and high-dose groups(0.6,1.2 g/kg).Except for the normal group,the eczema model was established in other groups by applying the 2,4-dinitrochlorobenzene solution to the hair removal area to sensitize it;they were given relevant drugs intragastrically,once a day,for consecutive 2 weeks.After medication,the skin thickness of rats was measured,and the eczema area and severity index(EASI)scoring and pathology scoring were performed;the structure of gut microbiota was analyzed by 16S rDNA sequencing.The contents of SCFA(including acetic acid,propionic acid,and butyric acid)in feces were determined by gas phase-mass spectrometry.The expressions of signal transduction and activator of transcription 3(STAT3)in the colon and skin were detected.RESULTS RZZY significantly alleviated skin swelling and ulceration and reduced EASI,skin thickness and pathological score in eczema rats(P＜0.05).RZZY significantly increased ACE,Sobs and Shannon indexes(P＜0.05)and promoted the microbiota of eczema rats to return to normal structure.RZZY increased the abundance of Firmicutes and Lachnospira,Lactobacillus and Alistipes,while decreased the abundance of Bacteroidetes and Bacteroides.RZZY significantly increased the contents of propionic acid and butyric acid in the feces of eczema rats(P＜0.05),while significantly down-regulated the expressions of STAT3 protein in the colon and skin(P＜0.05).CONCLUSIONS RZZY can improve SCFA levels by regulating gut microbiota,thereby inhibiting STAT3 expression,playing an anti-inflammatory role,and improving skin inflammation in eczema rats.
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