Content determination and chemometric analysis of 8 components in Smilax glabra
OBJECTIVE To provide reference for Smilax glabra quality control.METHODS UPLC method was established to simultaneously determine the contents of 5-O-caffeoylshikimic acid,neoastilbin,astilbin,neoisoastilbin,isoastilbin,engelitin,resveratrol and isoengelitin in 20 batches of S.glabra from different areas(No.S1-S20).The quality evaluation of 20 batches of samples was performed by chemometrics;the differential biomarkers that affected the quality of S.glabra were screened.RESULTS The measured 8 components had good linear relationship within the range of measured concentration(r≥0.999 6).RSDs of precision,repeatability and stability tests(24 h)were all lower than 2.00%(n=6).The average recoveries varied between 97.60%and 106.40%(RSDs were all lower than 2.00%,n=6).Cluster analysis showed that the samples produced in Zhejiang(S1-S5)and Jiangxi(S6-S10)were clustered into one category;the samples produced in Hunan(S11-S15)were clustered into one category;the samples produced in Yunnan(S16-S20)were clustered into one category.Principal component analysis showed that the first two principal components could represent 85.60%information of 8 components in S.glabra.Partial least squares-discriminant analysis showed that variable importance projection values of 5-O-caffeoylshikimic acid,astilbin,isoastilbin and neoastilbin were all greater than 1.CONCLUSIONS There are differences in the contents of the above 8 components in S.glabra from different origins;5-O-caffeoylshikimic acid,astilbin,isoastilbin and neoastilbin may be differential markers affecting the quality of S.glabra.
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