Study on the fingerprint and active ingredients contents of Denghong buyang huanwu granules
OBJECTIVE To study the fingerprint of Denghong buyang huanwu granules(DBHG),screen the quality markers,and establish the method for content determination of active ingredients.METHODS HPLC method was adopted.The fingerprints of 10 batches of DBHG(S1-S10)were established by using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(2012 edition),and similarity evaluation was also performed.Traditional Chinese medicine pieces attribution analysis,cluster analysis(CA),principal component analysis(PCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were conducted for common peaks,and quality biomarkers were screened based on variable importance projection(VIP)values＞1.The contents of 10 batches of samples were determined by the same HPLC method,such as salidroside,tyrosol,calycosin-7-O-beta-D-glucoside,scutellarin and calycosin.RESULTS A total of 25 common peaks were obtained in the fingerprints for 10 batches of samples and 6 common peaks were identified,i.e.salidroside,tyrosol,paeoniflorin,calycosin-7-O-beta-D-glucoside,scutellarin,calycosin.Their similarities were greater than 0.9,and 10 common peaks of them were unique components of Erigeron breviscapus.DBHG could be clustered into 2 categories by using CA and PCA;S4-S5,and S7 could be clustered into one category and other samples could be clustered into one category.The corresponding components of peaks 16(scutellarin),12,15(calycosin-7-O-beta-D-glucoside),13(paeoniflorin),and 14 were quality markers.The average contents of salidroside,tyrosol,calycosin-7-O-beta-D-glucoside,scutellarin and calycosin were 1.64,0.45,0.31,0.73,0.15 mg/g in 10 batches of samples.CONCLUSIONS HPLC fingerprint for DBHG and a method for determining the contents of five active ingredients including salidroside are successfully established.Five quality markers have been screened.It can be used for the quality control of the preparation.
万方数据
维普
