OBJECTIVE To establish the fingerprints of Tianma jiannao granules(TJG)and the method for content determination to evaluate the quality of TJG comprehensively combined with chemometric analysis.METHODS High-performance liquid chromatography(HPLC)was used to establish the fingerprints of 13 batches(S1-S3)of TJG and determine the contents of inosine,gastrodin,parishin B and parishin E.Cluster analysis,principal component analysis,and orthogonal partial least squares-discriminant analysis were performed using SPSS 20.0 and SIMCA 18 software;using variable importance projection(VIP)value greater than 1 as a criterion,marker components that affected quality were screened.RESULTS A total of 28 common peaks were identified in the 13 batches of TJG with similarities greater than 0.9,and 7 common peaks were identified,which were gastrodin,p-hydroxybenzyl alcohol,parishin B,parishin E,rhynchophylline,inosine and salidroside.The 13 batches of TJG were clustered into 3 categories,S1-S2,S8-S10 and S12 were clustered into one category;S3 and S7 were clustered into one category;S4-S6,S11 and S13 were clustered into one category.VIP of inosine was greater than 1.The contents of inosine,gastrodin,parishin B and parishin E were 62.637-176.677,17.821-37.642,5.748-16.077 and 5.660-13.510 μg/g.CONCLUSIONS The established HPLC fingerprints and content determination method are stable,reliable and highly reproducible,which can be used to evaluate the quality of TJG in combination with chemometric analysis.Inosine may be a marker component that affects the quality of TJG.There are differences in the quality of 13 batches of TJG.
维普
万方数据
