高致病性猪繁殖与呼吸综合征活疫苗(TJM-F92株)安全性和免疫保护效果研究
Safety and efficacy of an attenuated vaccine of highly pathogenic porcine reproductive and respiratory syndrome virus TJM-F92 strain
冷雪 1李真光 1夏铭崎 2徐晓琴 1武华2
作者信息
- 1. 中国农业科学院特产研究所特种经济动物分子生物学国家重点实验室,吉林长春130112
- 2. 华威特(北京)生物科技有限公司,北京100085
- 折叠
摘要
为进一步研究高致病性猪繁殖与呼吸综合征(HP-PRRS)活疫苗(TJM-F92株)的安全性和免疫保护效果,本研究将HP-PRRS弱毒株TJM-F92与耐热保护剂混合制备疫苗,将该疫苗以每头猪2 mL(106.4 TCID50/mL)的剂量接种4周龄～5周龄仔猪进行安全性试验;并以每头猪1 mL (105.0 TCID50/mL)的剂量免疫仔猪28 d后,利用同源强毒株(TJ株)攻毒进行保护效力检测.此外,分别采用103.5 TCID50/mL、104.0 TCID50/mL、1045 TCID50/mL和105.0 TCID50/mL的剂量,每头猪接种1 mL,通过攻毒试验确定疫苗最小免疫剂量.结果表明:疫苗接种后,试验猪无不良反应,免疫组攻毒保护率为80％～100％,每头份疫苗病毒含量为104.5 TCID50时对强毒攻击的保护率可达100％.本研究表明,该疫苗对猪安全,免疫保护效果良好,对HP-PRRS的防控将起到较大的推动作用.
Abstract
To evaluate the safety and efficacy of the attenuated highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS) vaccine strain of TJM-F92 derived from the HP-PRRS virus (HP-PRRSV) TJ strain,the lyophilized attenuated HP-PRRS vaccine was prepared with heat stable reagent,and the vaccine was inoculated into the 4-5 week old piglets for safety test at a dose of 2 mL (106.4 TCID50/mL) and for the protection test at a dose of 1 mL (1050 TCID50),challenged with HP-PRRSV TJ strain at 28 days post vaccination.In addition,the minimum immunization dose (MID) was also determined in piglets inoculated with the vaccine at the dose of 103.5 TCID50,104.0 TCID50,104.5 TCID50,and 1050 TCID50,respectively.The results revealed that the pigs inoculated with high dose of the vaccine (2× 106.4 TCID50) did not show any clinical signs.Furthermore,the vaccine provided 80％ to 100％ protection to pigs against the HP-PRRSV challenging and the MID was 1045 TCID50.These results indicated that the vaccine is safe,effective,and potential to be used for HP-PRRS prevention and control.
关键词
高致病性猪繁殖与呼吸综合征/弱毒活疫苗/安全性/免疫保护效果Key words
pathogenic porcine reproductive and respiratory syndorme/attenuated live vaccine/safety/efficacy引用本文复制引用
基金项目
农业公益性行业科研专项(201003060-04)
吉林省科技发展计划项目(20130522088JH)
出版年
2014
NETL
NSTL
维普
万方数据