To better understand molecular and evolutionary characteristics of the complete genome of goose-origin novel duck reovirus prevalence in Guangxi Province,liver,spleen,lungs and kidney samples from three sicked geese were collected.The samples were detected for Muscovy duck reovirus(MDRV)and novel duck reovirus(NDRV)by established fluorescence quantificative RT-PCR assay(RT-qPCR),and the positive NDRV sample was used as a template for complete genome amplification and sequencing.Subsequently,the sequence similarity was analyzed by BioEdit software,N-glycosylation sites prediction of σC protein was performed by NetNGlyc 1.0 online software,and phylogenetic and recombination analysis was conducted with Mega 7.0 and RDP and SimPlot software,respectively.One NDRV-positive sample was detected with RT-qPCR assay,and the complete genome sequence of G-NDRV-GX-2022 was acquired after amplification,sequencing and splicing.The genome length of the G-NDRV-GX-2022 strain was 23353bp,including 10 segments,L1-L4,M1-M3 and S1-S4.BLAST analysis showed that the highest nucleotide similarity to 10 genome segments of G-NDRV-GX-2022 strain ranged from 91.2%-98.4%,and these strains were derived from Guangdong,Fujian,Shandong,Zhejiang and Hubei Provinces.The hosts of these NDRVs were wild ducks,Muscovy ducks and mallard ducks,suggesting that genome sources of G-NDRV-GX-2022 strain were diverse.N-glycosylation sites prediction of σC protein was 121NLTS124,the same as NDRV strains.Phylogenetic analysis showed that σ series genes of G-NDRV-GX-2022 strain belonged to the NDRV cluster.The phylogenetic tree based on σB,σNS showed that the NDRV reference strain is continuous and uninterrupted in the evolution,while the tree based on σA,σC showed discontinuous evolution for the NDRV reference strain.None associated with prevalence area and time,indicating that genetics was diverse.Recombination signal was detected in the M3 gene of the G-NDRV-GX-2022 strain.The major parent was the SD-12 strain of wild duck with a similarity of 95.4%,and the minor parent was the GRV-GD2020 strain of goose reovirus with a similarity of 94.3%.Recombination of reovirus strains occurred between wild birds and poultry,suggesting that it could provide conditions for the generation of novel reovirus strains in the future.This study enriches the genetic information of aquatic bird-origin reovirus and provides a database for molecular genetic research on avian reovirus.
NETL
NSTL
维普
万方数据
