A real-time RT-PCR detection method was established for detecting equine arteritis virus.Primers and probes were designed and synthesized within highly conserved region ORF7 of the EAV genome sequence.The annealing temperature was 61℃and a final concentration of primers and probes were 0.6μmol/L after optimizing the reaction system.There was a well linear relationship between the dilution series of EAV cRNA(1.6×107 copies/μL-1.6×102 copies/μL)and Ct values(y=-2.68x+32.88,R2=0.9927).This method was specific for detecting EAV and had no cross reaction with horse Taylor wom,horse herpesvirus type 1,horse herpesvirus type 4,and horse influenza virus.The sensitivity could reach at 160 copies/μL of EAV cRNA.The inter-group and intra-group variable coefficient of this method were both within 2.0%.234 clinical samples were tested by this real-time RT-PCR method and a method from previous research.The above two methods had consistent positive rate of detection(14.1%,33/234),This method can be used for the early detection of equine viral arteritis virus infection,which provides workable solutions for the prevention and control of equine viral arteritis.
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