To evaluate the effect of cichoric acid on gut microbiota imbalance in piglets infected with porcine deltacoronavirus(PDCoV).In this study,colon content samples from cichoric acid group,cichoric acid+challenge group,challenge group and blank group were selected for 16S rRNA gene high-throughput sequencing(Illumina MiSeq),and the diversity of gut microbiota and composition of bacterial flora in each group were analyzed using bioinformatics software.Alpha diversity analysis showed that the diversity and abundance of colonic flora in the infected group were the lowest,and the flora was unbalanced.Compared with the infected group,the diversity and abundance of colonic flora in the chicory+infected group increased.Beta diversity analysis showed that the colony structure of the cichoric acid+challenge group was similar to cichoric acid group and blank group,but different from that of the infected group.Venn analysis at phylum,family,genus and OUT level showed that among the four experimental groups,the colonic flora in cichoric acid group had the highest abundance,the challenge group had the lowest colonic flora,and the colonic flora in cichoric acid+challenge group was similar to that of the blank group.The analysis of the bacterial community composition showed that the abundance of Firmicutes,Lactobacillaceae and Lactobacillus in challenge group increased compared with blank group,cichoric acid group and cichoric acid+challenge group,while the abundance of Bacteroidota decreased.The bacterial species difference analysis showed that the bacterial abundance of Synergistota was significantly increased in the blank group and chicoric acid group,and the bacterial abundance of Ruminococcaceae was the lowest in the infected group.These results suggest that oral administration of chicoric acid followed by PDCoV reinfection in piglets can improve the imbalance of gut microbiota caused by PDCoV infection and restore the diversity and richness of intestinal flora.This study provides a theoretical basis for the improvement of gut microbiota imbalance caused by PDCoV infection.
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