In order to explore the genetic evolutionary characteristics and haplotypes of Hyalomma anatolicum in Aiding Lake Town of Turpan(abbreviated as Turpan region)in Xinjiang,China,this study extracted 10 individual DNA samples from Turpan of China that have been morphologically identified as H.anatolicum.The universal primers for the 16S rRNA gene of the dominant Hyalomma species in Xinjiang of China designed by our laboratory were used to further identify the above H.anatolicum by PCR and followed by sequencing.DNAMAN V 5.0 software was used to analyze the homology of 16S rRNA genes of the 10 H.anatolicum from Turpan with those from Xinjiang(Awati)of China,Pakistan,and India.The phylogenetic tree of the 16S rRNA genes of H.anatolicum and those from the aforementioned was constructed by MEGA X software to analyze the phylogenetic and genetic diversity of the tick species.The PCR results showed that 388bp target gene fragments were amplified from all 10 samples of H.anatolicum.The BLAST comparison results showed that all 10 samples were H.anatolicum.The homology analysis of the 16S rRNA gene sequences showed that the highest homology among the 10 samples collected in this study was 99.57%,and the homology with the samples from Pakistan,India,and Xinjiang(Awati strain MG651939)of China,was 99.55%,99.17%,and 99.11%,respectively.The genetic evolution analysis of the 16S rRNA gene showed that tick 1,3,9 and 10 from Turpan region,Xinjiang of China,were clustered into a small branch with a relatively close genetic distance,and tick 2,4,and 8 from Pakistan,India,and Xinjiang of China,were clustered into a large branch with a relatively close genetic distance.It is suggested that H.anatolicum from Turpan region of China in this study may come from Pakistan.DNA SP V5.0 software was used to count the haplotype number(Np),haplotype diversity(Hd)and nucleotide diversity(pi)of the tick in Turpan and the aforementioned countries and regions.Popart v1.7 software was used to construct a haplotype network diagram of the tick in Turpan and the aforementioned areas.The haplotype statistical results showed that the Np of the tick from four different countries and regions was 8,namely Hap_1-Hap_8,respectively,Hd was 0.572 and pi was 0.01156.Compared with the other three regions,the highest Np of the tick in Turpan region in China was 7,Hd was 0.911 and the pi was 0.02593.The haplotype network diagram of the tick from the four countries and regions showed that only Hap_1 was found in the four countries and regions,while the haplotypes Hap_2 to Hap_8 were found in the other three regions.The H.anatolicum in Turpan of China not only shares haplotypes Hap_1,but also has haplotypes Hap_3-Hap_8.The above results indicated that H.anatolicum in Turpan region of China may originate from Pakistan and its genetic diversity was relatively rich.This study provides a reference basis for understanding the species identification and genetic evolutionary relationship among tick populations.
NETL
NSTL
万方数据
