In order to understand the epidemic status and genetic variation of porcine Getah virus(GETV)in China from 2021 to 2022,a total of 2512 clinical samples collected from 16 provinces were detected by fluorescence quantitative RT-PCR,and epidemiological surveys were analyzed by province and region.The E2 gene was amplified by RT-PCR and cloned into pMD18-T vector for sequencing.Then the homology and amino acid variation were analyzed by DNAStar software,and the phylogenetic tree of E2 gene was generated by MEGA6.0 software for further genotype and evolutionary relationship analysis.The results showed that GETV was detected in pig samples from 7 provinces,and the positive rate was 3.34%(84/2512).Among them,Shanxi province and Heilongjiang Province for the first time detected GETV in pig herds.Among pigs at different growth stages,piglets had the highest detection rate(9.52%).Homology analysis showed that the nucleotide homology among the 32 E2 genes of Porcine-origin GETV ranged from 98.3%to 100%,and the nucleotide homology with the Malaysian prototype strain of GETV MM2021 was 94.4%to 94.9%,respectively.Amino acid variation analysis showed that nine amino acid sites only found in porcine-derived strains compared with the reference strains.Genetic evolution analysis showed that all 32 strains belonged to subgroup III,which formed a new evolutionary branch compared with the early Chinese M1 strain,Korean QIAG9301 strain,and Japanese Kochi strain within subgroup III.This study revealed the current situation of GETV infection and the genetic variation of epidemic strains in China,and provides basic data for molecular biology research and disease prevention and control strategies of the virus.
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万方数据
