To investigate the effects of Alizarin(Alizarin,AZ)on intestinal flora and learning and memory ability of APP/PS1 double transgenic mice(APP/PS1 mice),the APP/PS1 mice were divided into model group(APP/PS1 group),alizarin groups(AZ Low,Middle,and High group)and positive control group(Donepezil group),and the APP/PS1 negative mice were used as blank control(Control group).After 4 weeks of continuous administration of AZ,the learning and memory abilities of mice in each group were evaluated by Morris water maze,and the changes of intestinal flora in feces of mice were detected by 16S rRNA gene sequencing.The results showed that compared with the control group,the escape latency of mice in APP/PS1 group prolonged significantly(P＜0.05),the number of platform crossing and the quadrant residence time decreased significantly(P＜0.01,P＜0.05),and the diversity of intestinal flora and the colony composition changed significantly.After intervention with AZ,the escape latency of mice shortened significantly(P＜0.05),the number of platform crossing and the quadrant residence time increased significantly(P＜0.05),AZ could effectively increase the diversity of intestinal flora and change the composition of microorganisms in APP/PS1 mice.For AZ group,the abundance of Bacteroides increased and the relative abundance of Bacteroides decreased at the phylum level;the relative abundance of Muribaculaceae and Lachnospiraceae bacteria increased and the abundance of Prevotellaceae,norank_o_Clostridia_UCG-014,Ruminococcaceae and other bacteria decreased at the family level;the relative abundance of Lactobacillus significantly increased at the genus level,and the dominant bacteria in AZ group were screened as o_Lactobacillales,f_Lactobacillaceae and g_Lactobacillus.Further function prediction analysis through PICRUSt2 found that the function of AZ regulating flora was related to inflammation-related signal pathway.This study revealed for the first time that AZ can improve the learning and memory ability of APP/PS1 mice by regulating intestinal flora,and its mechanism is related to inflammatory signal pathway,laying a foundation for the clinical application of AZ.
AlizarinAPP/PS1 double transgenic miceintestinal floralearning and memory ability
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