To explore the target of pulsatilla decoction(BTWT)in preventing Escherichia coli infections in broilers,the targets of BTWT were predicted in this study through the HERB2.0 database,and the related targets of E.coli infection were screened using the disease database Gene cards,and the intersection targets of the drug and the disease were identified.STRING online analysis platform was used to construct the protein-protein interaction(PPI)network diagram and screen the core targets of BTWT for inhibition of E.coli infection,the GO function of proteins and KEGG signaling pathway analyses were carried out,and molecular docking validation was carried out for the obtained core targets.The results showed that 95 active ingredients,462 disease targets,25 intersecting targets of drug-disease interactions,and 22 core targets were screened for BTWT.GO analysis yielded 49 functional entries,including 41 biological processes(e.g.,macrophage activity,immune response,and inflammatory response),4 cellular components(e.g.,extracellular space,cytoplasm),and 4 molecular functions(e.g.,interferon-gamma receptor binding),and KEGG pathway analysis yielded 11 signaling pathways,involving C-type lectin receptor signaling pathway,Toll-like receptor signaling pathway,apoptosis,NOD-like receptor signaling pathway,etc.The molecular docking results showed that the free energy of γ-aminobutyric acid docked with SOD1,Qinpiin docked with IL-10,and quercetin docked with IFN-γ were all<0 kcal/mol.Sixty healthy broilers were selected and randomly divided into three groups of 20 in each group,which were healthy control group(Group C),infected group(Group M)and BTWT group(Group PD).The Group C and M were fed with basal diet,and the Group PD were fed with basal diet supplemented with 10%BTWT for 7 days.Groups M and PD were administrated orally with E.coli O78 strain(1.09×108 cfu)at 8 days,and after 2 days,the protection rate and immune organ index of BTWT on E.coli-infected broilers were calculated,the contents of cytokines and antioxidant indexes in serum were determined by ELISA kit,and the morphological changes of the jejunum were observed under light microscope.The results showed that BTWT provided certain protection against E.coli infection in broilers with a protection rate of 20%compared with Group M.The fasciola index and thymus index in Group PD increased,but the difference was not significant(P>0.05),and the spleen index significantly decreased(P<0.05);the serum IgA content significantly increased(P<0.05),and the IL-10,TNF-α and IFN-γ levels significantly decreased(P<0.05).Meanwhile,the levels of SOD and GSH-PX in serum significantly increased(P<0.05),the level of MDA significantly reduced(P<0.05),and the damage of jejunum was alleviated.The above results indicated that pulsatilla decoction provided a good prevention against E.coli infection in broilers,and may alleviate the damage caused by E.coli infection by regulating the levels of SOD1,IL-10 and IFN-γ,offering a new idea for the in-depth study of the mechanism of action of pulsatilla decoction in the prevention of E.colidisease.
维普
万方数据
