In order to understand the molecular characteristics of complete genome of a novel goose-origin astrovirus(GoAstV)prevalent in Guangxi,three tissue samples(kidney,liver and spleen)from goose suspected of GoAstV infection were collected and detected using the fluorescence quantification RT-PCR assay(RT-qPCR).Positive GoAstV samples were used as template for the ampli-fication and sequencing of the complete genome sequences.The similarity analysis was conducted using BioEdit software,while epitope sites and N-glycosylation sites prediction of Cap protein were predicted using Bepipred and NetNGlyc 1.0 online software.Phylogenetic and recombination analysis were performed with Mega 7.0 and RDP 5.Three GoAstV positive samples were identified and the genome sequence of GoAstV-GXHZ01-2022 strain was obtained.The genome length was 7149bp,consisting of a 143bp 5'untranslated region(UTR),a 6929bp open reading frame(ORF)and a 77bp 3'UTR.Similarity analysis based on the nucleotide sequences of ORF1a,ORF1b and ORF2 of GoAstV-GXHZ01-2022 showed 53.0%-53.4%,63.2%-63.5%,and 48.2%-48.4%similarity to classical GoAstV strains,and 97.0%-99.6%,98.1%-99.8%,and 97.5%-99.8%similarity to novel GoAstV reference strains.Epitope sites prediction of Cap protein of GoAstV-GXHZ01-2022 identified sites at aa6-aa16,aa20-aa66,aa80-aa86.N-glycosylation sites prediction were identified at 81NSTD84,401NTTG404,464NITF467 and 531NTST534.Phylogenetic analysis based on nucleotide sequences of ORF,ORF1a,ORF1b and ORF2 genes indicated that GoAstV-GXHZ01-2022 and novel GoAstV reference strains belonged to the same branch,most closely related to AAstV-3,followed by AAstV-1 and AAstV-2 strains.Recombination signal was detected in the ORF1a and ORF2 genes of GoAstV-GXHZ01-2022 strain,with the major parent being the AstV/AH01/Goose/0512/18 strain from Anhui Province(98.6%similarity),and the minor parent being AstV/HB02/Goose/0310/19 strain from Henan Province(99.1%similarity).The findings suggested that the GoAstV-GXHZ01-2022 strain shares the highest similarity to novel GoAstV reference strains,exhibits an identical genetic trend,and shows evidence of recombination.This study enriches the genomic database and provides valuable information on GoAstV strains prevalent in Guangxi.
维普
万方数据
