Infectious laryngotracheitis virus(ILTV)constitutes a major threat to the poultry industry worldwide,particularly in China.Using the chicken liver hepatocellular carcinoma(LMH)cell line infected with ILTV-LJS09 as the experimental model,this study conducted a whole-genome transcriptome analysis and found that the MAPK pathway is the most significantly activated pathway in LMH cells in response to ILTV infection.Addtionally western blot detection identified significant activation of host cell p38/MAPK by ILTV infection.To investigate the impact of pharmacological p38/MAPK activation on ILTV infection,two activators,Dehy-drocorydaline(DHC)and Phorbol 12-myristate 13-acetate(PMA),were employed in LMH cells.Fluorescence quantitative PCR was employed to detect the copy number of the virus genome,the 50%tissue culture infectious dose was used to determine viral titers,Fluorescence quantitative PCR was used to detect the transcription level of metabolism-related genes,and high-content imaging technology combined with Annexin V-FITC/propidium iodide double staining was used to detect cell apoptosis,thereby exploring the effect of pharm-acological activation of p38/MAPK on ILTV infection.The results of virus-specific fluorescence quantitative PCR detection showed that both DHC and PMA significantly reduced the replication of the ILTV genome(P<0.05).The detection of 50%tissue culture infectious dose showed that DHC and PMA also significantly reduced the proliferation of infectious ILTV virus particles(P<0.05).The results of fluorescence quantitative PCR detection showed that neither DHC nor PMA had a significant impact on the overall expression of metabolic genes in infected cells.The analysis of high-content imaging showed that DHC and PMA significantly intensified the apoptosis of ILTV-infected cells(P<0.05).In summary,the results of this study show that both DHC and PMA can effectively inhibit ILTV infection by intensifying the apoptosis of ILTV-infected cells without affecting the expression of metabolic genes in infected cells.This study underscores the potent antiviral effects of pharmacological p38/MAPK activation in an in vitro ILTV infection model.These insights not only introduce a novel therapeutic avenue for ILTV but also offer valuable prospects for the prevention and management of other herpesviruses.
infectious laryngotracheitis virusp38 mitogen-activated protein kinasetranscriptional profileapoptosis
维普
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