To investigate the preventive and therapeutic effects of matrine(MT)on diarrhea induced by Clostridium perfringens type A in mice,56 healthy 8-week-old male Kunming mice were evenly divided into four groups:control group(PBS),bacterial suspension group(Cp A 0.2mL/mouse,1×109 cfu/mL),treatment group,and prevention group.In the treatment group,mice were intragastric administered MT(30mg/kg)for 9 days after Clostridium perfringens type A gavage for 7 days.In the prevention group,mice were simultaneously intragastric administered with Clostridium perfringens type A bacterial suspension and MT for 16 days.After weighing the mice and collecting blood in each group,serum was separated,and inflammatory cytokines in the serum were detected using the ELISA method.Ileal tissues and intestinal contents were collected to determine the number of Clostridium perfringens type A by PCR and the amount of α-toxin released by ELISA kit.The results showed that the body weight of mice in the treatment and prevention groups increased significantly.In the bacterial suspension group,the intestinal villi of the mice were shed,and the number of lymphocytes and red blood cells increased.However,in the prevention and treatment groups,the infiltration of inflammatory cells in the intestine decreased,the structure of intestinal villi gradually recovered,and the intestinal tissue damage caused by inflammation was repaired.ELISA results showed no significant difference in the levels of IL-1β,IL-6,TNF-α,and MAPK8 in the serum of mice in the pre-vention group compared with the control group(P>0.05).The levels of IL-6 and MAPK8 in the treatment group were significantly higher than those in the control group(P<0.05).PCR results showed no significant difference in the amount of Clostridium perfringens type A in the intestinal contents of mice in the treatment and prevention groups compared with the bacterial suspension group(P>0.05).However,compared with the control group,there was a significant inhibitory effect on the content of α-toxin produced by Clostridium perfringens type A.These results indicate that MT can inhibit the release of α-toxin by Clostridium perfringens type A,restore the mucosal immune barrier of the ileum in mice,and reduce intestinal inflammation caused by Clostridium perfringens.This study provides a theoretical basis for the application of MT in the treatment and prevention of enteritis caused by Clostridium perfringenstype A.
MatrineClostridium perfringenstype Amiceinhibitionin vitrotreatment and prevention
维普
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