To investigate the pathogens of diarrhea and death of youn pigeons in a pigeon farm in Kashgar.In this study,71 stool samples from diarrhea breeding pigeons and 43 liver,kidney,spleen and intestine samples from dead young pigeons were collected from February to March 2023,and the bacteria were isolated and cultured,and the Klebsiella pneumoniae(Kp)was amplified and sequenced by PCR using 16S rRNA and ITS(16S rDNA-23S rDNA internal transcription interval sequence).The virulent gene of Kp was detected by PCR.The drug sensitivity of Kp was determined by using a Kirby-Bauer disk diffusion method and the drug resistance genee were detected by PCR.Epidemiological typing was performed by enterobacterial repetitive intergenic consensus-polymerase chain reaction(ERIC-PCR),and Multi-Locus Sequence Typing(MLST)was implemented to detect the sequence type(ST).The pathogenicity of Kp was detected by mouse pathogenicity test.The results showed that a total of 83 Kp isolates were isolated from 114 samples.The detection rates of virulence gene uge,mrkD,rmpA,wabG,ureA and magA were 85.5％-98.7％,and the detection rates of fimHwere 1.2％.The drug resistance rates of 83 Kp isolates to amoxicillin,ampicillin,cefotaxime and ceftazidime ranged from 73.0％to 100％,that to ceftriaxone,amikacin,levo floxacin,tetracycline,streptomycin,azithromycin,florfenicol,polymyxin B and gentamicin ranged from 1.2％to 25.0％,among of which 4 isolates were multiple drug resistance.The prevalence of drug resistance genes blaSHV-11(72.2％),blaCTX-M-1G(43.3％)were high,while the carrying rates of blaCTX-M-9G(14.5％,12/83)and tet(A)(6.0％,5/83)were low.blaTEM,blaCTX.M-2G,aac,aadAl,strA,strB,tet(E),tet(G)and mph(A)were not detected.ERIC-PCR and MLST typing showed that the Kp genetic relationship between breeding pigeons and young pigeons were closed relatively,and ST23 was the main type.The median lethal dose(LD50)for mice was 3.6× 103cfu/mL.In this study,Kp was isolated from diarrhea breeding pigeons and dead young pigeons for the first time.It was highly pathogenic,carried rich virulence genes,and was seriously resistant to β-lactam drugs.This provided a basis for the rational use of drugs to effectively control Kp infection in meat pigeons.
维普
万方数据
